Sawant, M SZhang, S CWang, Q YWang, Y J2009-05-282009-05-282004-08-03Sawant MS, Zhang SC, Wang QY, Wang YJ. Production of interspecies chimeras by transplanting rosy barb (Puntius conchonius) embryonic cells to zebrafish (Danio rerio) embryos. Indian Journal of Experimental Biology. 2004 Aug; 42(8): 830-6http://imsear.searo.who.int/handle/123456789/62834Establishment of a cell-mediated gene transfer system has potential as a new breeding technique for commercially valuable fishes. As an important step toward developing an inter-species chimera, cells from blastula-stage embryos of rosy barb (Puntius conchonius) were transplanted into zebrafish (Danio rerio) blastula-stage embryos to observe the development of the recipient. From the total of 473 transplants obtained only a fraction of 13 chimeras appeared perfectly normal after one month. Over two in normal 13 chimeras showed some characters from the donor cells with scarce pigmentation. This is the first successful inter-species study on zebrafish by using blastula cell transplants from rosy barb both belonging to the same family cyprinidae.engAnimalsBase SequenceBreedingChimera --embryologyCyprinidae --embryologyDNA Primers --geneticsGene Transfer TechniquesPhenotypePigmentationStem Cell TransplantationZebrafish --embryologyJournal Article