Poth Thirakhupt2011-02-222011-02-222010-01-132010-01-13Vajira Medical Journal; Vol. 51 No. 3 September - December 2007; 189-194http://imsear.searo.who.int/handle/123456789/133189Abstract Effect of Punica granatum Linn. Peel Extracts on the Growth of Common Bacteria Causing Infection in Burn Patients Poth            Thirakhupt     MD Department of Surgery, BMA Medical College and Vajira Hospital Objective: To study the effect of Punica granatum Linn. peel extracts on the growth of common bacteria causing infection in burns patients. Study design: Experimental study. Materials: Punica granatum Linn. peel extracts and common bacteria causing infection in burn patients. These bacteria consisted of Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa, Acenitobacter baumannii, Klebsiellla pneumoniae prepared from microbiology laboratory, BMA Medical College and Vajira Hospital during May to August 2007. Methods: The extract was prepared by solvent extraction technique using hexane and methanol. Then, the extract was dissolved in distilled water to make a concentration of 300 mg/ml. for testing. Each bacteria was prepared in three agar plates. Paper disc with the extracts was laid in the culture plate of the bacteria and incubated at 37 for 24 hours. Main outcome measure: The diameter of inhibition zone (clear zone) was recorded in cm. Results: There were clear zones only in Staphylococcus aureus and MRSA plates. The average diameter of clear zones were 2.1 ± 0.1 and 2.0 ± 0.2 cm. On the contrary, there were no clear zones in Pseudomonas aeruginosa, Acenitobacter baumannii, Klebsiellla pneumoniae plates. Conclusion: Punica granatum Linn peel extract at the concentration of 300 mg/ml inhibited the growth of Staphylococcus aureus and MRSA. Vajira Med J 2007 ; 51 : 189 - 194en-USFaculty of Medicine, Vajira Hospital, University of Bangkok Metropolis, Bangkok, ThailandOriginal Articles