Fruit Peel from Astrocaryum aculeatum G. Mey. AS Substrate for Lipases Production by Aspergillus niger A2B1

Diniz, Nayara Fátima Lazamethda Silva, Rossicléa GonzagaOliveira, Luciana AiresCaldas, Waldireny Rochade Souza, Érica SimplícioFiglioulo, RobertoOliveira, Juliana Gomes de Souzade Souza, João Vicente Braga2019-12-062019-12-062018-11Diniz Nayara Fátima Lazameth, da Silva Rossicléa Gonzaga, Oliveira Luciana Aires, Caldas Waldireny Rocha, de Souza Érica Simplício, Figlioulo Roberto, Oliveira Juliana Gomes de Souza, de Souza João Vicente Braga. Fruit Peel from Astrocaryum aculeatum G. Mey. AS Substrate for Lipases Production by Aspergillus niger A2B1. Biotechnology Journal International. 2018 Nov; 22(1): 1-82456-7051http://imsear.searo.who.int/handle/123456789/188618The fruit of Astrocaryum aculeatum G. Mey. are consumed by population in the Brazil Northern resulting fruit peels. These peels are rich in lignocellulose and fat. The present study investigated peels from the Astrocaryum aculeatum G. Mey. as a substrate for lipases production by solid state bioprocess. To reach this objective: 1) we isolated fungi from peels from the fruit of Astrocaryum aculeatum G. Mey; 2) we screened the isolates for lipase production (screening in petri dish and screening in submerged bioprocess), 3) we investigated the production of lipases by using peels from Astrocaryum aculeatum G. Mey as substrate. The isolates belonged to the genera Aspergillus (16), Penicillium (3) and Fusarium (1). These strains were submitted to petri dishes and submerged fermentation for lipases production, these experiments resulted in the selection of five strains belonging to the Aspergillus genera. The lipases produced by these five strains performed enzymatic transesterification; however, the lipases from the strain Aspergillus niger A2B1 produced the highest ester content. The utilisation of fruit peel from Astrocaryum aculeatum G. Mey. as the main substrate, fruit peel from Astrocaryum aculeatum G. Mey. oil (13%), moisture (70%) and 75 h of incubation were the optimal conditions identified for the production of lipases by Aspergillus niger A2B1(17.42 U/g) in solid state bioprocess (SSB).Tucumã peelbioprocesslipasesenzymatic transesterification.Fruit Peel from Astrocaryum aculeatum G. Mey. AS Substrate for Lipases Production by Aspergillus niger A2B1Journal ArticleIndiaLaboratório de Micologia, Instituto Nacional de Pesquisas da Amazônia (INPA), Brazil.Laboratório de Micologia, Instituto Nacional de Pesquisas da Amazônia (INPA), Brazil.Laboratório de Micologia, Instituto Nacional de Pesquisas da Amazônia (INPA), Brazil.Instituto de Saúde e BIotecnologia-ISB, Universidade Federal do Amazonas, Brazil.Escola Superior de Tecnologia, Universidade do Estado do Amazonas, Brazil.Coordenação de Tecnologia e Inovação Instituto Nacional de Pesquisas da Amazônia (INPA), Brazil.Laboratório de Micologia, Instituto Nacional de Pesquisas da Amazônia (INPA), Brazil.