Celestine, U. O.Jude, A. I.Keneolisa, A. F.Chigozie, U.- ugo J.Chijioke, O. R.Maduabuchi, O. R.Ifeanyi, U. F.Ogbonna, O. R.Assumpta, A. C.2023-07-142023-07-142023-04Celestine U. O., Jude A. I., Keneolisa A. F., Chigozie U.- ugo J., Chijioke O. R., Maduabuchi O. R., Ifeanyi U. F., Ogbonna O. R., Assumpta A. C.. Anti-inflammatory Assessment of Methanol Extract of Acalypha ciliata Leaves and It’s Leucocyte Mobilization in Adult Wistar Rats. European Journal of Medicinal Plants. 2023 Apr; 34(4): 22-292231-0894http://imsear.searo.who.int/handle/123456789/219546Aim: The study's objective was to examine the anti-inflammatory activity and leucocyte mobilization of a methanol extract of leaves from Acalypha ciliata in adult Wistar rats. Study Design: Egg albumin-induced edema was used for anti-inflammatory test and leucocyte mobilization test was carried out to check for total leucocyte cout and differentials. Place and Duration of the Study: This original study was conducted between January and June 2013 at the Department of Biochemistry, Faculty of Biological Sciences, University of Nigeria Nsukka. Methology: The pulverized leaf of Acalypha ciliata was extracted using cold maceration, and the Treas and Evans technique of phytochemical screening was used. The anti-inflammatory study was conducted using the method of Winter et al., and the acute toxicity study was assessed using Lorke's method. ANOVA was used to statistically examine the collected data. Results: Preliminary phytochemical screening revealed the presence of flavonoids, alkaloids, soluble carbohydrates, phenols, glycosides, saponins, terpenoids and steroids. Flavonoids, Alkaloids, and Soluble carbohydrates appeared in abundant concentration (2199.76±10.81, 2141.6±7.583, 913±21.06). Acute toxicity tests showed no toxicity and mortality at doses up to 5000 mgkg-1. Anti-inflammatory study revealed that group treated with 100, 200 and 400 mg/kg b.w of extract significantly (P =0.05) and in a dose dependant manner decreased in TLC when compared with normal control. Moreover, the group treated with 100,200,400 mg/kg b.w of extract and reference drug(Indometacin) mobilized the leucocyte at the rate of 36, 22, 12 and 62 % respectively. A significant (P = 0.05) reduction in the mean paw oedema was observed for all the treatment groups from 1 hour to 5 hours when compared to the toxic group. Conclusion: As a result of this study's findings, it can be concluded that methanol extract of the Acalypha ciliata leaf has anti-inflammatory characteristics. High dosages of the extract resulted in a better decrease of oedema and an increase in leukocyte mobilization in response to agar suspension than low doses. Many anti-inflammatory herbs and substances alter inflammatory reactions by hastening the breakdown of or reversing the effect of the inflammatory mediators. The plant's anti-inflammatory properties could potentially result from the interaction of different photochemical substances present. The findings suggest that, if used, the plant may serve as a source of anti-inflammatory compounds.Acalypha ciliateanti-inflammatorypaw oedemaleukocyte migrationJournal ArticleIndiaDepartment of Pharmacology, Faculty of Pharmaceutical Sciences, Enugu State University of Science and Technology, Agbani, NigeriaDepartmentof Biochemistry, Faculty of Biological Science University of Nigeria,Nsukka,NigeriaDepartment of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Enugu State University of Science and Technology, Agbani Enugu,NigeriaCollege of Natural Science, Micheal Okpara University of Agriculture, Umudike, NigeriaDepartment of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, Enugu State University of Science and Technology, Agbani Enugu,NigeriaUburu Bioresources Biotechnology Development Agency, Abuja, NigeriaDepartment of Pharmacology and Therapeutics, Faculty of Clinical Medicine, Enugu State University of Science and Technology, Nigeria