Pandey, B DKarabatsos, NCropp, BTagaki, MTsuda, YIchinose, AIgarashi, A2009-05-272009-05-271999-03-01Pandey BD, Karabatsos N, Cropp B, Tagaki M, Tsuda Y, Ichinose A, Igarashi A. Identification of a flavivirus isolated from mosquitos in Chiang Mai Thailand. The Southeast Asian Journal of Tropical Medicine and Public Health. 1999 Mar; 30(1): 161-5http://imsear.searo.who.int/handle/123456789/35812The Southeast Asian Journal of Tropical Medicine and Public Health.A virus isolate, ThCAr105/92, from a pool of mosquitos, Culex tritaeniorhynchus, collected in Chiang Mai, Thailand in 1992, appeared to be a member of the genus Flavivirus of the family Flaviviridae, based on the reverse transcription polymerase chain reaction (RT-PCR) using flavivirus cross-reacting primer pairs, electron microscopic examination, and serological tests. However, RT-PCR using Japanese encephalitis (JE) virus-specific primers showed that the isolate was different from JE virus. Sucrose density gradient sedimentation of the virus replicated in C6/36 cells indicated that the virus is relatively unstable in the infected culture fluids at 37 degrees C. Antibody prepared against this virus and a virus seed for the isolate were tested by cross neutralization against a panel of flaviviruses and the results showed that the new isolate was a distinct subtype of Tembusu virus.engAnimalsCulex --virologyDNA, Viral --analysisFlavivirus --classificationMicroscopy, ElectronNeutralization TestsReverse Transcriptase Polymerase Chain ReactionSerotypingThailandVirologyVirus CultivationJournal Article