Tiwari, RashmiGhildiyal, SnehaGaur, PoojaFatima, TanzeemDhole, Tapan NSrivastva, Janmejai KUpadhyay, ShivbratAtam, Virendra2023-06-172023-06-172023-01Tiwari Rashmi, Ghildiyal Sneha, Gaur Pooja, Fatima Tanzeem, Dhole Tapan N, Srivastva Janmejai K, Upadhyay Shivbrat, Atam Virendra. The relationship between MMP-2 rs243865, MMP-9 rs398242 and CXCL-12 rs1801157 gene polymorphisms with Japanese encephalitis disease and disease outcome in North Indian population. Journal of Vector Borne Diseases . 2023 Jan; 60(1): 65-730972-9062http://imsear.searo.who.int/handle/123456789/216918Background & objectives: Japanese encephalitis virus (JEV) is one of the most important causes of acute and uncontrolled inflammatory disease in Asia. Matrix metalloproteinases (MMPs) and chemokines play a detrimental role in the host response to JE disease, aetiology, and disease outcome. Evidently, MMPs are widely circulated in the brain and regulate various process including microglial activation, inflammation, blood-brain barrier disruption as well as affects central nervous system (CNS). The present study was to assess the association of single nucleotide polymorphisms of MMP-2, MMP-9 and chemokine (CXCL-12/SDF1-3’) in the north Indian population. Methods: We performed case-control study comprising of 125 patients and 125 healthy controls in north Indian population. Genomic DNA was extracted from whole blood and gene polymorphism have been determined by PCR-RFLP method. Results: MMP-2, MMP-9 and CXCL-12 gene was not significantly associated with JE disease, but homozygous (T/T) genotype of MMP-2 was statically associated with disease outcome (p=0.05, OR=0.110). A/G and G/G genotype of CXCL-12 was significantly associated with severity of disease. (p=0.032, OR=5.500, p=0.037, OR= 9.167). The serum level of MMP-2 was observed significantly increased in JE patients with homozygous (T/T) genotype whereas increased MMP-9 level was associated with heterozygous genotype. Interpretation & conclusion: MMP-2, MMP-9 and CXCL-12 gene polymorphism were not associated with JE susceptibility, but MMP-2 may be contributed to disease protection. CXCL-12 was associated with disease severity. In our concern this is the first report from northern India.Japanese EncephalitisMMP-2MMP-9CXCL-12Gene polymorphismJournal ArticleIndiaSanjay Gandhi Post Graduate Institute of Medical Sciences Uttar Pradesh, Lucknow, IndiaAmity Institute of Biotechnology, Amity University Uttar Pradesh, Lucknow, IndiaEra ’s Lucknow Medical College and Hospital, Lucknow, Uttar Pradesh, India King George Medical University, Lucknow, Uttar Pradesh, India