Sangtawesin, WHijikata-Okunomiya, AOpartkiattikul, NWongtiraporn, WLuenee, PButthep, PKitaguchi, HFucharoen, SFunahara, Y2009-05-272009-05-271997-03-01Sangtawesin W, Hijikata-Okunomiya A, Opartkiattikul N, Wongtiraporn W, Luenee P, Butthep P, Kitaguchi H, Fucharoen S, Funahara Y. Surface and total tissue factor activity of endothelial cells. The Southeast Asian Journal of Tropical Medicine and Public Health. 1997 ; 28 Suppl 3(): 164-6http://imsear.searo.who.int/handle/123456789/31338The Southeast Asian Journal of Tropical Medicine and Public Health.With a technic that was developed by us, we found that normal human umbilical vein endothelial cells (HUVEC) in culture characteristically had very little tissue factor (TF) activity either on the surface or in the cells which had been disrupted. In the presence of endotoxin (E. coli O26:B6), a trigger for thrombosis in septicemic patients, we could not detect an increased TF activity of HUVEC on its surface. However, an increase in TF (total TF) was detected after disruption of the cells. The increase in total TF was dose-dependent. Endotoxin at the concentration of 10 micrograms/ml caused around 5 fold increase in total TF activity compared to that of HUVEC in the absence of endotoxin.engCells, CulturedEndothelium, Vascular --chemistryEndotoxins --diagnostic useHumansThromboplastin --analysisJournal Article