Naik, Kamal SatgondaGupta, Rajkumar B.2023-08-282023-08-282022-07Naik Kamal Satgonda, Gupta Rajkumar B.. Comparative in vitroantimicrobial study of Panchavalkal and modified Panchavalkalin three different extract solvents: a study protocol. International Journal of Advances in Medicine. 2022 Jul; 9(7): 825-8292349-39332349-3925http://imsear.searo.who.int/handle/123456789/225850Background: Charak, Sharangdhar, Kashyap, BhavaPrakasha described Panchavalkala, is a combination of five astringent barks viz., Vata (Ficus bengaenesisLinn.), Udumbara (Ficus glomerataRoxb.), Ashvattha (Ficus religiosaLinn.), Parisha (Thespesia populaneaSolandexcorrea) and Plaksha (Ficus slacor Buch-Ham.). In Ayurveda sometimes substitute plants are described to work better for a particular medical condition. Such type of alternative drug suggested by BhavaMishra and Kaiyadeva Nighantu for classical Panchavalkala is Shirish (Albezzia lebbeckLinn.) in place of Parisha. Such type of altered Panchavalkal is called as modified Panchavalkal. Both Panchavalkals are essentially important in acute, chronic ulcers, wound healing and reproductive health concern women’s diseases like leucorrhea, irregular or heavy bleeding etc. So, these Panchavalkals at three different extract solvents i.e.,water extract, alcoholic extract and hydro alcoholic extractin comparison to standard anti-microbial drugs will be studied.Methods: Panchavalkal and modified Panchavalkalin water, alcohol and hydro alcoholic extract will be subjected to a test antimicrobial property by agar well diffusion method. Results will be assessed on the basis of minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and the zone of inhibition (ZOI) of plant extracts against the microorganism that will be measured.Conclusions: Conclusion will be drawn on the basis of results obtained about anti-microbial activity of Pachavalkal and modified Panchavalkal in three different extracts.Trial registration:It is taken, Ref. No. MGACHRC/IEC/February-2021/193-APanchavalkalModified PanchavalkalAnti-microbial activityJournal ArticleIndiaDepartment of Dravyaguna, LRPAMC, Islampur, Sangli, Maharashtra, IndiaDepartment of Dravyaguna, MGACH and amp RC, Datta MegheInstitute of Medical Sciences, Wardha, Maharashtra, India