Reddy, I MohanRao, A G AppuRao, M S Narasinga2015-07-202015-07-201982-06Reddy I Mohan, Rao A G Appu, Rao M S Narasinga. Isolation and characterization of the 7S protein from glanded cottonseed (Gossypium herbacium). Journal of Biosciences. 1982 Jun; 4(2): 197-208.http://imsear.searo.who.int/handle/123456789/160141The major protein from glanded cottonseed has been isolated in a homogeneous form. Its S20, w value at 1% protein concentration is 6S in 1 Μ NaCl solution. It contains 1% carbohydrate and is free from phosphorus, gossypol (bound or free) and nucleic acid impurities. It consists of atleast seven non-identical subunits. The protein has an ultraviolet absorption maximum at 278 nm and fluorescence excitation and emission maxima at 280 nm and 325 nm respectively. Optical rotatory dispersion and circular dichroism measurements indicate that the protein consists predominantly of β-structure and random coil. The observed near-ultraviolet circular dichroic bands can be attributed to tyrosine, phenylalanine and tryptophan residues of the protein.enIsolationcharacterizationcottonseed protein7S proteingossypolArticle