Enbavelan, P.A.Kavitha, S.Selvaraj, P.Vairamuthu, S.Annal Villi, R.2025-05-122025-05-122024-08Enbavelan P.A., Kavitha S., Selvaraj P., Vairamuthu S., Annal Villi R. . Prevalence and Alterations in Metabolic Indices and Cowside Test in Subclinical Ketosis of Prepartum Transition Dairy Cows . Journal of Animal Research. 2024 Aug; 14(4): 257-2632249-66292277-940Xhttps://imsear.searo.who.int/handle/123456789/247429Subclinical ketosis is the overproduction of ketone bodies in the blood that causes decreased milk production and reproductive effectiveness without exhibiting any overt clinical signs of ketosis during the transition period of dairy cows. Therefore, the study was conducted to find the alterations of metabolic parameters and the usefulness of cow side tests in the early detection of subclinical ketosis in prepartum itself so that the occurrence of clinical ketosis can be prevented. The prevalence of subclinical ketosis in prepartum was 16.66 percent (36/216) among prepartum dairy cows. Cow side test revealed an increase in BHBA level, a decrease in glucose value, and the presence of ketonuria compared with the respective days of a control group. A significant increase in the concentration of serum metabolites such as non-esterified fatty acid (NEFA) and beta hydroxybutyric acid (BHBA), along with increased body condition score, decreased reticulo-rumen mortality and rumen fill score and reduced rumen protozoa score were recorded. The serum biochemical changes recorded were a decrease in serum glucose, triglyceride, cholesterol direct bilirubin, calcium, and phosphorous and an increase in BUN, Aspartate aminotransferase (AST). An elevation of acute phase protein Haptaglobin was also recorded in subclinical ketosis cows. This levels can be used as risk indicators to predict the occurrence of subclinical ketosis in prepartum transition cows.PrepartumSubclinical ketosisNEFABHBACowsideASTJournal ArticleIndiaDepartment of Veterinary Medicine, Veterinary College and Research Institute, Salem, Tamil Nadu, INDIADepartment of Veterinary Clinical Medicine, Madras Veterinary College, Chennai, Tamil Nadu, INDIAVeterinary University Peripheral Hospital, Madhavaram, Chennai, Tamil Nadu, INDIACentralized Clinical Laboratory, Madras Veterinary College, Chennai, Tamil Nadu, INDIAVeterinary University Training and Research and Training Centre, Krishnagiri, INDIA