Sharma, ManshiJangir, O PJhajharia, SunitaSingh, SumerSingh, VijayNagal, Amit2013-01-022013-01-022010-01Sharma Manshi, Jangir O P, Jhajharia Sunita, Singh Sumer, Singh Vijay, Nagal Amit. Trans-differentiation of iris pigmented epithelial cells of Euphlyctis cyanophlyctis tadpoles into lens in vitro. Indian Journal of Experimental Biology. 2010 Jan; 48(1): 17-25.http://imsear.searo.who.int/handle/123456789/144091Meshed pigmented iris epithelium along with neural retina of tadpoles of the frog Euphlyctis cyanophlyctis were found to undergo dedifferentiation and subsequently transdifferentiate into lens in culture medium. During lag period, depigmentation (dedifferentiation) occurred in many cells. When culture became confluent 3-4 weeks after seeding tiny lens like structures differentiated from foci of cultured pigmented iris epithelium cells. The percentage of lens formation was higher in vitamin A treated cases. The culture system appears to be a suitable for investigating the changes occurred during trans-differentiation of pigmented epithelial cells into lens.enFrogLensPigmented epithelial cellsTrans-differentiationAnimalsCell Transdifferentiation --drug effectsIris --cytologyIris --drug effectsLarva --cytologyLarva --growth & developmentLens, Crystalline --cytologyLens, Crystalline --drug effectsLens, Crystalline --growth & developmentPigment Epithelium of Eye --cytologyPigment Epithelium of Eye --drug effectsRanidae --anatomy & histologyRanidae --growth & developmentTissue Culture TechniquesVitamin A --pharmacologyArticle