Joshi, A RSinha, SDil-Afroz,Sulaiman, I MBanerji, A KHasnain, S E1996-12-012009-05-271996-12-012009-05-271996-12-01Joshi AR, Sinha S, Dil-Afroz , Sulaiman IM, Banerji AK, Hasnain SE. Alterations in brain tumor DNA detected by a fingerprinting probe. Indian Journal of Biochemistry & Biophysics. 1996 Dec; 33(6): 455-7http://imsear.searo.who.int/handle/123456789/26769We used a novel DNA fingerprinting probe O-chi-1 (ref. 1) to detect differences in the hybridization pattern of brain tumor DNA and paired normal tissue of a given individual. Representatives of meningiomas (two), glioblastoma multeforme (three) and astrocytoma (one) were studied. Alterations, which included amplification as well as the loss of a normal band in tumor DNA, were observed in four of the six tumours. While the increased intensity of a band can be taken to imply increased copy number, the disappearance of bands could either be due to loss of DNA sequence or rearrangement resulting in different sized bands.engAstrocytoma --geneticsBlotting, SouthernBrain Neoplasms --geneticsDNA Fingerprinting --methodsDNA ProbesDNA, Neoplasm --chemistryDeoxyribonucleases, Type II Site-Specific --metabolismElectrophoresis, Agar GelGlioblastoma --geneticsHumansMeningioma --geneticsJournal Article