Kheyrodin, Hamid2016-11-222016-11-222015-09Hamid Kheyrodin. Introduction of Electrophoresis Process. World Journal of Clinical Pharmacology, Microbiology and Toxicology. 2015 Sept; 1(3): 14-21.2454‐1729http://imsear.searo.who.int/handle/123456789/178681Electrophoresis is the process of moving charged molecules in solution by applying an electric field across the mixture. Electrophoresis is often classified according to the presence or absence of a solid supporting medium or matrix through which the charged molecules move in the electrophoretic system. The fundamental driving force of electrophoresis is the voltage applied to the system. Gel electrophoresis is a laboratory method used for a variety of protocols including sequencing, RFLP analysis, marker analysis, DNA fingerprinting and DNA purification. It is based on two key principles: 1) DNA has an overall negative charge (due to the phosphate backbone) and thus will migrate towards a positive charge. 2) A gel acts as a sort of microscopic sieve; smaller DNA fragments will travel through the gel more rapidly than larger fragments. Thus DNA can be separated based on the length (size) of the DNA segment In this study we worked in Desert laboratory in semnan university for analysis effect of temperature on gle electrophoresis. We conducted that method of electrophoresis used to separate proteins or nucleic acids (DNA and RNA) across a temperature at 4°C for 10-15 minutes. poured gel at 4°C for 10-15 minutes OR let sit at room temperature for 20-30 minutes, until it has completely solidified.enElectrophoresisGel ElectrophoresisDNA MoleculesArticle