Ahmed, HSAmir, DEMuhaisen, HMHSebak, MAbdelghany, TMElmaidomy, AHAmin, EOwis, AIOrabi, MAAAfifi, N.2024-11-302024-11-302024-06Ahmed HS, Amir DE, Muhaisen HMH, Sebak M, Abdelghany TM, Elmaidomy AH, Amin E, Owis AI, Orabi MAA, Afifi N. . New bisnorsesquiterpene from Glottiphyllum linguiforme and its antimicrobial, cytotoxic, and antioxidant activities. Journal of Applied Pharmaceutical Science. 2024 Jun; 14(6): 97-1042231-3354https://imsear.searo.who.int/handle/123456789/237690Natural product research is a cornerstone in the drug discovery process. Many inspiring chemical moieties with new skeletons and potent biological activity are commonly reported from natural sources. Chromatographic examination of the alcoholic extract of Glottiphyllum linguiforme aerial furnished four compounds, including ?-sitosterol (1), ?-sitosterol-3-O-?-D-glucopyranoside (2), and 1,1? biuracil (3), and a new compound, tectoionyl A 3-O-sodium sulfate (4). The new structure was elucidated using high-resolution mass and intensive 1D and 2D NMR spectroscopic analyses. Quantitative analysis of G. linguiforme extract revealed considerable contents of total phenolic and total flavonoid. A DPPH antioxidant assay indicated moderate activity (0.026 mg acid equivalent antioxidant capacity/g dry extract). Antimicrobial activity testing of the pure compounds indicated noticeable antibacterial activity of compound 2 against Escherichia coli, Salmonella enterica, and Pseudomonas aeruginosa. In addition, compounds 1–4 displayed mild cytotoxic effects against human breast adenocarcinoma (MCF7) and hepatocellular carcinoma (HepG2) cell lines, and the normal (Vero) cells at the tested concentration (100 ?g/ml), as compared to the standard cytotoxic drug, doxorubicin. This study is the first to estimate phenolics and flavonoid contents, discover constituents by phytochemical isolation, and examine cytotoxicity and antimicrobial properties of G. linguiforme. The unusual nitrogenous compound 1,1?-biuracil (3) and the sulfate derivative (4) may evidence chemotaxonomic importance.Glottiphyllum linguiformetectoionyl A-3-O-sodium sulfateantioxidantantimicrobialcytotoxicity.Journal ArticleIndiaPharmacognosy Department, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, EgyptPharmacognosy Department, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, EgyptDepartment of Chemistry, Faculty of Science and Arts, Najran University, Sharurah, Saudi ArabiaMicrobiology and Immunology Department, Faculty of Pharmacy, Beni-Suef University, EgyptPharmacology and Toxicology Department, Faculty of Pharmacy, Al-Azhar University, Cairo Egypt; Pharmacology and Toxicology Department, Faculty of Pharmacy, Heliopolis University for Sustainable Development, Cairo, EgyptPharmacognosy Department, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, EgyptDepartment of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, Qassim University, Buraydah, Saudi ArabiaPharmacognosy Department, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt; Pharmacognosy Department, Faculty of Pharmacy, Heliopolis University for Sustainable Development, Cairo, EgyptDepartment of Pharmacognosy, College of Pharmacy, Najran University, Najran, Saudi ArabiaPharmacognosy Department, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt