Dhawan, RMukerjee, RChaturvedi, U CKhare, S D2009-05-282009-05-281995-11-01Dhawan R, Mukerjee R, Chaturvedi UC, Khare SD. Flow cytometric analysis of T4:T8 splenic cell during dengue virus infection of mice. Indian Journal of Experimental Biology. 1995 Nov; 33(11): 816-23http://imsear.searo.who.int/handle/123456789/60743The present study was undertaken to investigate the effect of dengue type 2 virus (DV) and DV-induced cytokines (CF and CF2) on T lymphocyte subpopulations of spleen by flow cytometry. Following DV-ic inoculation in mice the percent number of CD4+ and CD8+ lymphocytes in the spleen was reduced, the peak reduction in both was observed on the 6th day post-inoculation (p.i.). Intravenous inoculation of CF or CF2 in mice also decreased the percent number of CD4+ as well as CD8+ T lymphocytes subpopulation in the spleen, the maximum reduction being observed at 1 and 2 hr, respectively. The reduction in T lymphocyte subpopulation by CF and CF2 was found to be dose dependent. Thus, the alterations of T lymphocyte subpopulations during DV infection are mediated via cytokines.engAnimalsCD4-CD8 RatioCytokines --biosynthesisDengue --immunologyFlow CytometryMiceMice, Inbred StrainsSpleen --immunologyJournal Article