Wongratanacheewi, SSermswan, R WAnuntagool, NSirisinha, S2009-05-272009-05-272001-06-09Wongratanacheewi S, Sermswan RW, Anuntagool N, Sirisinha S. Retrospective study on the diagnostic value of IgG ELISA, dot immunoassay and indirect hemagglutination in septicemic melioidosis. Asian Pacific Journal of Allergy and Immunology. 2001 Jun; 19(2): 129-33http://imsear.searo.who.int/handle/123456789/37222Published by the Allergy and Immunology Society of Thailand.Three serological methods for diagnosis of melioidosis were compared with the culture method currently used as the "gold standard". The diagnostic values of the serological methods were evaluated retrospectively in 306 patients residing in an endemic area. The enzyme-linked immunosorbent assay (ELISA), using affinity purified antigen for detecting specific IgG antibody, showed a slightly higher specificity (86.0%) than the dot immunoassay (DOT) (84.0%) and both were superior to indirect hemagglutination (IHA) (72.0%). The sensitivity of DOT (96.4%) and ELISA (85.7%) were considerably higher than that of IHA (50.0%). The primary benefit of the high negative predictive value of both ELISA (96.4%) and DOT (99.0%) in an area of high prevalence is the ability to rule out most of the non-melioidosis patients.engBacteremia --bloodBurkholderia pseudomallei --isolation & purificationEnzyme-Linked Immunosorbent AssayFalse Positive ReactionsHemagglutination TestsHumansImmunoblottingImmunoglobulin G --bloodMelioidosis --bloodPredictive Value of TestsRetrospective StudiesSensitivity and SpecificityThailandComparative Study