Xiaoming, WuHao, ChenGaoyue, ZhangJianhui, WuWei, ZhuYanqin, GuYi, He2020-11-182020-11-182019-09Xiaoming Wu, Hao Chen, Gaoyue Zhang, Jianhui Wu, Wei Zhu, Yanqin Gu, Yi He. MiR-212-3p inhibits cell proliferation and promotes apoptosis by targeting nuclear factor IA in bladder cancer. Journal of Biosciences. 2019 Sep; 44(4): 1-80250-59910973-7138http://imsear.searo.who.int/handle/123456789/214423Accumulating evidence suggest that microRNAs play crucial roles in the development and progression of bladder cancer(BC). Here, we found that miR-212-3p was significantly down-regulated and negatively correlated with nuclear factor IA(NFIA) in human BC tissues. Bioinformatics analysis predicted that NFIA was a target gene of miR-212-3p. Then BC celllines, T24 and J82 cells were transfected with miR-212-3p mimics or siNFIA to obtain miR-212-3p overexpression or NFIAknockdown cell lines, respectively. Quantitative real-time PCR was used to determine the expression of miR-212-3p andNFIA. Western blot analysis was utilized to detect NFIA expression. MTT assay showed either miR-212-3 overexpression orNFIA knockdown significantly inhibited the BC cell proliferation. Double staining with Annexin V-APC and 7-AAD showedthe total number of apoptotic BC cells were remarkably increased after miR-212-3p overexpression or NFIA knockdown.Collectively, our results indicated that miR-212-3p targeting NFIA might serve as a promising target for BC.Apoptosisbladder cancermiR-212-3pNFIAproliferationJournal ArticleIndiaDepartment of Urology, the First Hospital of Jiaxing, Jiaxing, Zhejiang Province, People’s Republic of China