Mbah, M.Akpan, S. S.Bebia, G. P.Tangban, E. E.Bassey, E. E.2023-07-142023-07-142023-02Mbah M., Akpan S. S., Bebia G. P., Tangban E. E., Bassey E. E.. A Bacteriological of Make-up Tools Used in Calabar Metropolis, Cross River State, Nigeria. Annual Research & Review in Biology. 2023 Feb; 38(2): 28-342347-565Xhttp://imsear.searo.who.int/handle/123456789/219420Sharing of make-up tools by multiple users is a possible means of transfer of fungal infections, such as dermatophytoses. With abrasion on the skin, it is possible for HIV, Hepatitis B virus, Spirochaetes and other pathogens to be carried via contaminated make-up tools, from one person to another. Using sterilized swab sticks, surfaces of make-up tools (sponge and brush) were cleansed. Materials deposited on the swab sticks were cultured aerobically on Chocolate and Cysteine Lactose Electrolyte Deficient Agar plates overnight at 37oC. After overnight aerobic incubation, the culture plates were read macroscopically for growth. Bacterial isolates were subjected to microscopic examination using Gram’s staining technique and biochemical tests (e.g. coagulase, catalase, oxidase, and motility). Results showed that the predominant bacterial growths obtained from the make-up tools were Staphylococcus aureus (51.5%), Coagulase-negative Staphylococcus (25.8%), Klebsiella pneumoniae (6.2%), and Pseudomonas aeruginosa (16.5%). The different genera of bacteria were harvested from make-up brushes (66.2%) and make-up sponges (85.7%). There was no bacterial growth from 33.8% and 14.3% of make-up brushes and make-up sponges respectively. There was no statistical difference in terms of bacterial growth between the two tools (brush and sponge) sampled (P>0.05). This study has shown that there is a moderate possibility for the transfer of bacterial organisms (both skin flora and pathogens) from one person to another, through make-up tools, in our local communities. It is hereby recommended that health education talks should be carried out regularly among beauticians to encourage them to use disposable make-up tools with disinfectants.Bacteriacalabarmake-uptoolsurveyJournal ArticleIndiaUniversity of Calabar, Calabar, P.M.B 1115, Calabar, Nigeria.