Sasi, P KRaj, R K1976-07-012009-06-011976-07-012009-06-011976-07-01Sasi PK, Raj RK. Colorimetric determination of piperazine. Indian Journal of Physiology and Pharmacology. 1976 Jul-Sep; 20(3): 180-2http://imsear.searo.who.int/handle/123456789/107942A sensitive method for the quantitative determination of piperazine is described. The method is precise and responds linearly from 25 g to 500 mug and above of the material. The procedure is based on the formation of a complex of piperazine with reineckate in neutral or acid medium. The complex can be separated by centrifugation. It is then dissolved in acetone and estimated at 530 nm in a colorimeter. Piperazine present in trichloroacetic acid extractrs of biological samples can also be estimated by this method.engAnimalsAscaris --analysisChromiumColorimetry --methodsHumansIndicators and ReagentsLiver --analysisPiperazines --analysisQuaternary Ammonium CompoundsThiocyanatesJournal Article