Siddiq, AAM, NM, AGS, SP, SMR, M.2025-05-122025-05-122024-12Siddiq A, AM N, M A, GS S, P S, MR M.. An Approach to Anti-Diabetic Potential of Putranjiva roxburghii Wall Leaves by In vitro Models . Journal of Pharmaceutical Research International. 2024 Dec; 36(12): 72-772456-9119https://imsear.searo.who.int/handle/123456789/247691Aim: To evaluate the antidiabetic potential of Putranjiva roxburghii leaves using In vitro models, focusing on their ability to inhibit key enzymes involved in carbohydrate metabolism. Study Design: The current research program is an attempt to screen In vitro anti-diabetic activity of ethanolic extract of Putranjiva roxburghii wall leaves by using – ? amylase inhibitory activity and starch iodine assay. Place and Duration of Study: Department of Pharmacology, SJM College of Pharmacy Chitradurga, between July to October. 2024 Methodology: In this study, the crude extract was obtained by using the soxhlet extraction method using 70% ethanol, The extract was used to analyse the antidiabetic activity using in-vitro models namely ?-amylase inhibition activity and starch iodine assay, all the experiments were performed in triplicates. The effect of leaf extract was compared with the standard drug metformin. Results: The results of ?-amylase inhibition activity showed that the extract inhibits the ? amylase enzyme in a concentration (0.3,0.6,0.9,1.2 and 1.5mg/ml) with a percentage inhibition range (6.02%-55.55%) for extract which is not significantly different as compared to metformin (16.12% - 69.89%). Similarly, for starch iodine assay the extract inhibits the ?-amylase enzyme in a concentration (same as above) with a percentage inhibition range (16.66%-75%) for extract which is not significantly different as compared to metformin (22.57% - 85.71%). Conclusion: From the above finding of the study, Putranjiva roxburghii wall leaf extract has the ability to inhibit ?-amylase enzyme and exhibits antidiabetic activity.Diabetes mellitusanti-diabetic?-amylasePutranjiva roxburghii wallstarch iodineJournal ArticleIndiaDepartment of Pharmacology, SJM College of Pharmacy, SJM Campus, Chitradurga-577502, Karnataka, IndiaDepartment of Pharmacology, SJM College of Pharmacy, SJM Campus, Chitradurga-577502, Karnataka, IndiaDepartment of Pharmacology, SJM College of Pharmacy, SJM Campus, Chitradurga-577502, Karnataka, IndiaDepartment of Pharmacology, SJM College of Pharmacy, SJM Campus, Chitradurga-577502, Karnataka, IndiaDepartment of Pharmacology, SJM College of Pharmacy, SJM Campus, Chitradurga-577502, Karnataka, IndiaDepartment of Pharmacology, SJM College of Pharmacy, SJM Campus, Chitradurga-577502, Karnataka, India.