Agarwal, Samarth KumarPraveen, GGupta, SaurabhTandon, RenuGupta, Sameer2012-08-062012-08-062011-07Agarwal Samarth Kumar, Praveen G, Gupta Saurabh, Tandon Renu, Gupta Sameer. In vitro evaluation of cytotoxicity of denture adhesives. Indian Journal of Dental Research. 2011 Jul-Aug; 22(4): 526-529.http://imsear.searo.who.int/handle/123456789/140066Aim: The aim of the study is to assess and compare the cytotoxicity of commercially available four denture adhesives ex-vivo. Materials and Methods: Four commercially available denture adhesives namely Metrodent powder, Fixon powder, Dentiro powder and Fixon cream were selected. Normal saline was used in control group. To evaluate the cytotoxicity of denture adhesives, macrophages were isolated from peritoneal cavity of Swiss albino mice and cell integrity/cell viability method was done by using trypan blue dye. Results: Viable cells were counted and subjected to statistical analysis. ANOVA, F and 't' test were performed, which showed statistically significant values (P < 0.001). The mean percentage of viable cells was highest in the control group (95%) and lowest in Fixon powder (55.66%), with Dentiro powder the mean percentage of viable cells was 63.66%, with Metrodent powder 67.6% while with Fixon cream it was 69.33%. Conclusion: All tested denture adhesives showed varied degree of cytotoxicity that is statistically significant. The degree of toxicity was more in Fixon powder followed by Dentiro powder and Metrodent powder with least in Fixon cream.enCell viabilitycytotoxicitydenture adhesivemacrophagesAdhesives --toxicityAnimalsCell CountCell SeparationCell Survival --drug effectsCells, CulturedColoring Agents --diagnostic useDenture RetentionMacrophages, Peritoneal --drug effectsMaterials TestingMiceTrypan Blue --diagnostic useArticle