Dwivedi, EktaKushwaha, RashmiJain, MiliYadav, GeetaVerma, S.P.Singh, U.SKumar, Ashutosh2020-09-242020-09-242019-07Dwivedi Ekta, Kushwaha Rashmi, Jain Mili, Yadav Geeta, Verma S.P., Singh U.S, Kumar Ashutosh. To Assess the Correlation between Bone Marrow Morphology and Immunophenotypic Findings in Patients of Chronic Lymphoproliferative Disorders (CLPD’s) and to Assess the Role of Flowcytometric Immunophenotyping in Diagnosis and Subclassification of CLPD’s. International Journal of Contemporary Medical Research . 2019 Jul; 6(7): g5-g112393-915X2454-7379http://imsear.searo.who.int/handle/123456789/202453Introduction: Chronic lymphoproliferative disorderrepresent clonal proliferation of morphologically andimmunophenotypically mature B or T cells characterized by alow proliferation rate and prolonged cell survival. Study aimedto assess the correlation between bone marrow morphologyand immunophenotypic findings in patients of ChronicLymphoproliferative Disorders (CLPD’s) and to assess therole of flowcytometric immunophenotyping in diagnosis andsubclassification of CLPD’s.Material and Methods: 48 newly diagnosed cases ofCLPD were included. After complete clinical evaluation theyunderwent marrow aspiration, biopsy and immunophenotypingby flowcytometry with selected panel of monoclonalantibodies.Results: On morphology 47.9% cases were CLL. In 52.1%non CLL cases , 4.2% were PLL , 2% case as LPL and45.8% cases were CLPD-unclassifiable. Commonest patternof marrow infiltration noted on trephine biopsy was diffuse inCLL, HCL-V, B-PLL and T-CLPD. On immunophenotyping95.8% cases were B-CLPD and 4.25% T-CLPD. CD5, CD22,CD23, FMC7 and SmIg were used as first line markersfollowed by CD 10, CD 25, CD103, CD38, CD138 andCyclin D1 (on biopsy sections) as second line markers. Finalimmunophenotypic diagnosis was CLL (54.2%), B-CLPDunclassified (29.2%), 4.1% each of LPL, MCL, T-CLPD and2% each of B-PLLand HCL-V.Conclusion: Concordance rate between morphologicaldiagnosis and immunophenotypic diagnosis was 79.17%.Hence, Flowcytometry is necessary for confirmationof diagnosis and to classify the CLPD cases which areunclassifiable by morphologyCLPDMorphology; CLPDImmunophenotype; CLPDFlowcytometryJournal ArticleIndiaJR, Department of Pathology, K.G.M.U, LucknowProfessor, Department of Pathology, K.G.M.U, LucknowAssistant Professor, Department of Pathology, K.G.M.U, LucknowAssistant Professor, Department of Pathology, K.G.M.U, LucknowAssociate Professor, Department of Clinical Hematology, K.G.M.U, LucknowProfessor, Department of Pathology, K.G.M.U, LucknowHead and Professor, Department of Pathology, K.G.M.U, Lucknow, India