Padma, PSetty, O H1997-06-012009-05-271997-06-012009-05-271997-06-01Padma P, Setty OH. Effect of administration of galactosamine hydrochloride on rat liver mitochondria. Indian Journal of Biochemistry & Biophysics. 1997 Jun; 34(3): 296-301http://imsear.searo.who.int/handle/123456789/27103The effect of galactosamine on liver mitochondrial functions was studied in vivo in rats at 12hr, 24hr and 36hr after the administration of the drug. State 3 respiration decreased significantly with both NAD+ linked and FAD linked substrates. Respiratory control ratio, an index of membrane integrity and P/O ratio which is a measure of phosphorylation efficiency decreased significantly. There was a significant decrease in the activities of NADH dehydrogenase, succinate dehydrogenase and cytochrome oxidase. A significant decrease was also seen on membrane potential, cytochrome aa3, cytochrome b, cytochrome c and on phospholipids of mitochondria. The observed mitochondrial dysfunctions were related to increased lipid peroxidation, which could cause loss of membrane integrity and a decreased rate of phosphorylation. It is proposed that increased lipid peroxidation was responsible for the inhibition on both oxidation and phosphorylation in mitochondria in galactosamine treated rats.engAnimalsCell Respiration --drug effectsCytochromes --analysisElectron Transport --drug effectsElectron Transport Complex IV --metabolismGalactosamine --pharmacologyLipid Peroxides --analysisLipids --analysisMitochondria, Liver --drug effectsNADH Dehydrogenase --metabolismPhosphorylationRatsSuccinate Dehydrogenase --metabolismJournal Article