Qi, WenjingLi, JinjiaoPei, XiaohuaKe, YueshuangBu, QingpanNi, Xiuzhen2020-11-182020-11-182020-02Qi Wenjing, Li Jinjiao, Pei Xiaohua, Ke Yueshuang, Bu Qingpan, Ni Xiuzhen. β-Actin facilitates etoposide-induced p53 nuclear import. Journal of Biosciences. 2020 Feb; : 1-80250-59910973-7138http://imsear.searo.who.int/handle/123456789/214331As a tumor suppressor, p53 preserves genomic integrity in eukaryotes. However, limited evidence is availablefor the p53 shuttling between the cytoplasm and nucleus. Previous studies have shown that b-actin polymerization negatively regulates p53 nuclear import through its interaction with p53. In this study, we found thatDNA damage induces both b-actin and p53 accumulation in the nucleus. b-actin knockdown impaired thenuclear transport of p53. Additionally, b-actin could interact with p53 which was enhanced in response togenotoxic stress. Furthermore, N terminal deletion mutants of p53 shows reduced levels of association with bactin. We further identified Ser15, Thr18 and Ser20 of p53 are critical to the b-actin: p53 interaction, whichupon mutation into alanine abrogates the binding. Taken together, this study reveals that b-actin regulates thenuclear import of p53 through protein–protein interaction.b-actinp53interactionDNA damagenuclear localizationJournal ArticleIndiaDepartment of Bioscience, Changchun Normal University, 677 Changji Northroad, Changchun 130032, Jilin, ChinaThe Key Laboratory of Molecular Epigenetics of MOE, Institute of Genetics and Cytology, School of Life Sciences, Northeast Normal University, Changchun 130024, Jilin, ChinaDepartment of Bioscience, Changchun Normal University, 677 Changji Northroad, Changchun 130032, Jilin, ChinaThe Key Laboratory of Molecular Epigenetics of MOE, Institute of Genetics and Cytology, School of Life Sciences, Northeast Normal University, Changchun 130024, Jilin, ChinaDepartment of Bioscience, Changchun Normal University, 677 Changji Northroad, Changchun 130032, Jilin, ChinaDepartment of Bioscience, Changchun Normal University, 677 Changji Northroad, Changchun 130032, Jilin, China