Yang, ZhaoweiQiao, LuChao, YangLiu, JuanDi, YanqingSun, JingZhang, JiebingHuang, LihongGuo, HonghuaHe, Chengyan2020-01-022020-01-022019-12Yang Zhaowei, Qiao Lu, Chao Yang, Liu Juan, Di Yanqing, Sun Jing, Zhang Jiebing, Huang Lihong, Guo Honghua, He Chengyan. High expression of nucleophosmin is closely related to the grade and invasion of colorectal cancer. Indian Journal of Biochemistry and Biophysics. 2019 Dec; 56(6): 420-4260975-09590301-1208http://imsear.searo.who.int/handle/123456789/190071This study explores the differential protein expression in the colorectal cancer (CRC) patients to validate a new biomarker for tumor progression. CRC tissues and their adjacent non-cancerous tissues were analyzed by two-dimensional LC/MS/MS. Nucleophosmin 1 (NPM1) was selected and confirmed its differential expression by Western blot. Immunohistological staining of NPM1 in tissues was performed to validate its correlation with clinicopathologic parameters of CRC patients. There were 39 candidates with significant difference between cancerous tissues and their adjacent non-cancerous tissues, which included 19 increased proteins and 20 decreased proteins in CRC samples. Especially, NPM1 was correlated with poor differentiation, and lymph node metastasis according to the analysis of patients’ clinicopathologic parameters. Increased expression of NPM1 can be as a critical biomarker for clinical diagnosis of tumor progression of CRC patients.Journal ArticleIndiaDepartment of Medical Laboratory and Clinical Diagnosis; 2Department of Digestive Internal Medicine; China-Japan Union Hospital of Jilin University, Jilin Sheng- 130 000, ChinaDepartments of Digestive Internal Medicine, Qian Wei Hospital of Jilin Province, Changchun- 130 000, ChinaDepartment of Geriatrics, China-Japan Union Hospital of Jilin University, Jilin Sheng- 130 000, China