Balne, P KBasu, SRath, SBarik, M RSharma, S2016-08-092016-08-092015-10Balne P K, Basu S, Rath S, Barik M R, Sharma S. Loop mediated isothermal amplification assay using hydroxy naphthol blue, conventional polymerase chain reaction and real-time PCR in the diagnosis of intraocular tuberculosis. Indian Journal of Medical Microbiology. 2015 Oct-Dec; 33(4): 568-571.http://imsear.searo.who.int/handle/123456789/176515This study is a comparative evaluation (Chi-square test) of a closed tube loop mediated isothermal amplification assay using hydroxy naphthol blue dye (HNB-LAMP), real-time polymerase chain reaction (PCR) and conventional PCR in the diagnosis of intraocular tuberculosis. Considering clinical presentation as the gold standard in 33 patients, the sensitivity of HNB-LAMP assay (75.8%) was higher (not significant, P value 0.2) than conventional PCR (57.6%) and lower than real-time PCR (90.9%). Specificity was 100% by all three methods. No amplification was observed in negative controls (n = 20) by all three methods. The cost of the HNB-LAMP assay was Rs. 500.00 and it does not require thermocycler, therefore, it can be used as an alternative to conventional PCR in resource-poor settings.enHydroxynaphthol blueloop mediated isothermal amplification assayMPB64ocular tuberculosisreal-time polymerase chain reactionArticle