Iyer, Srividhya VRamakrishnan, MukundKasbekar, Durgadas P2009-04-072009-06-022009-04-072009-06-022009-04-07Iyer SV, Ramakrishnan M, Kasbekar DP. Neurospora crassa fmf-1 encodes the homologue of the Schizosaccharomyces pombe Ste11p regulator of sexual development. Journal of Genetics. 2009 Apr; 88(1): 33-9http://imsear.searo.who.int/handle/123456789/114423The Neurospora crassa fmf-1 mutation exerts an unusual 'perithecium-dominant' developmental arrest; fmf-1 x fmf-1+ cross becomes arrested in perithecial development regardless of whether the mutant participates in the cross as the male or female parent. We localized fmf-1 to the LG IL genome segment between the centromere-proximal breakpoint of the chromosome segment duplication Dp(IL)39311 and the centromere. By mapping crossovers with respect to RFLP markers in this region we further localized fmf-1 to an approximately 34-kb-genome segment. Partial sequencing of this segment revealed a point mutation in the gene NCU 09387.1, a homologue of the Schizosaccharomyces pombe ste11+ regulator of sexual development. The fmf-1 mutation did not complement a NCU 09387.1 deletion mutation, and transformation with wild-type NCU 09387.1 complemented fmf-1. S. pombe Ste11 protein (Ste11p) is a transcription factor required for sexual differentiation and for the expression of genes required for mating pheromone signalling in matP and matM cells. If FMF-1 also plays a corresponding role in mating pheromone signalling in Neurospora, then protoperithecia in an fmf-1 x fmf-1+ cross would be unable to either send or receive sexual differentiation signals and thus become arrested in development.engFungal Proteins --geneticsFungal Proteins --metabolismGene Expression Regulation, FungalGenome, FungalModels, GeneticMutationNeurospora crassa --geneticsSchizosaccharomyces --geneticsSchizosaccharomyces pombe Proteins --geneticsTranscription Factors --geneticsTranscription Factors --metabolismJournal Article