Debnath, Sujit KumarSaisivam, SDash, Dillip KumarDebnath, Monalisha2015-12-142015-12-142015Debnath Sujit Kumar, Saisivam S, Dash Dillip Kumar, Debnath Monalisha. Development and validation of UV-spectrophotometric methods for quantitative estimation of Prothionamide in pure and pharmaceutical dosage forms. International Current Pharmaceutical Journal. 2015; 4(7): 402-404.2224-9486http://imsear.searo.who.int/handle/123456789/167999ABSTRACT UV Spectrophotometric method was developed and validated for the quantitative determination of Prothionamide in bulk drug and in pharmaceutical formulations. Prothionamide shows the maximum absorbance at 288 nm in phosphate buffer (pH 7.4). Prothiona-mide follows Beer’s law in the concentration range of 4-20 μg/ml (r2 = 0.999). The detection limit (DL) and quantitation limit (QL) were 0.406 and 1.229 μg/ml respectively. Accuracy and precision were found to be satisfactory. The developed methods were validated according to ICH guidelines. All the validation parameters were found to be satisfactory accordance with the standard values. Therefore, the proposed method can be used for routine practice for the determination of Prothionamide in assay of bulk drug and pharmaceutical formulationsenProthionamideUV-Spectrophometryvalidationantitubercular drugcontent determinationArticle