Zeng, FanyeYu, NingHan, YanyanAiniwaer, Julaiti2020-11-182020-11-182020-03Zeng Fanye, Yu Ning, Han Yanyan, Ainiwaer Julaiti. The long non-coding RNA MIAT/miR-139-5p/MMP2 axis regulates cell migration and invasion in non-small-cell lung cancer. Journal of Biosciences. 2020 Mar; : 1-120250-59910973-7138http://imsear.searo.who.int/handle/123456789/214312Non-small-cell lung cancer (NSCLC) is a complex disease which is influenced by multiple factors. Recentstudies demonstrated that long non-coding RNA (lncRNA) MIAT was involved in tumor metastasis. However,the underlying mechanism of MIAT in NSCLC remains largely unknown. In this study, MIAT, miR-139-5pand MMP2 expression were measured by quantitative reverse transcriptase PCR (QRT-PCR) or Westernblotting, respectively, and we found the expression of MIAT and MMP2 were elevated, while miR-139-5p wasdecreased in NSCLC tissues and cell lines. Transwell assay showed MIAT and MMP2 functioned as anoncogene to induce cell migration and invasion in NSCLC, but miR-139-5p served as a tumor suppressor inNSCLC to inhibit cell migration and invasion. Besides that, in vivo experiments also indicated MIAT deletioninhibited tumor growth. The relationship between miR-139-5p and MIAT or MMP2 was then confirmed byLuciferase reporter assay, and the results showed that MIAT directly interacted with miR-139-5p and miR-139-5p targetedly suppressed MMP2 in NSCLC cells. Furthermore, expression analysis showed that MIAT indirectly regulated MMP2 by sponging miR-139-5p. Finally, rescue assay suggested that miR-139-5p restorationreversed MIAT-overexpression-induced promotion on the migration and invasion of NSCLC cells. In conclusion, our results demonstrated that lncRNA MIAT modulated the migration and invasion of NSCLC byregulating miR-139-5p and MMP2.Long non-coding RNA MIATmetastasismiR-139-5pMMP2NSCLC cell linesxenograftsJournal ArticleIndiaDepartment of Second Oncology, Xinjiang Traditional Chinese Medicine Hospital, Xinjiang, ChinaDepartment of First Thoracic Surgery, Xinjiang Chest Hospital, Xinjiang, ChinaDepartment of Otolaryngology Head and Neck Surgery, Xinjiang Urumqi Eye and ENT Hospital, Xinjiang, ChinaDepartment of Thoracic Surgery, the First Affiliated Hospital of Xinjiang Medical University, Xinjiang, China