Detection of extended spectrum beta-lactamase from clinical isolates in Davangere.
dc.contributor.author | Sridhar Rao, P N | en_US |
dc.contributor.author | Basavarajappa, K G | en_US |
dc.contributor.author | Krishna, G Leela | en_US |
dc.date.accessioned | 2008-10-15 | en_US |
dc.date.accessioned | 2009-05-29T11:17:38Z | |
dc.date.available | 2008-10-15 | en_US |
dc.date.available | 2009-05-29T11:17:38Z | |
dc.date.issued | 2008-10-15 | en_US |
dc.description.abstract | Extended spectrum beta-lactamase enzymes (ESBLs) are enzymes that have the ability to hydrolyze oxyiminocephalosporins and infections by isolates producing them are often difficult to treat. A study to detect the presence of these enzymes in isolates was conducted by our hospital. A total of 207 non repetitive isolates were screened for resistance to any of five screening agents. Those with suspicious profiles were checked for ESBL production by double-disk approximation or a synergy test. The isolates were also subjected to a phenotypic confirmation test as recommended by CLSI (formerly NCCLS). Various cephalosporins-beta-lactamase inhibitor combinations were also tested. Of the 204 (98.5%) screen-positive isolates, only 126 (61.7%) were identified as ESBL producers. Of these, 26.1% of the isolates were positive by using the double-disk synergy test (DDST) method alone, 13.4% were positive using the method recommended by CLSI, and 60.3% of the isolates were positive by both the DDST and CLSI methods. We also report a high percentage of resistance to cefoxitin (96.8%) indicating changes in porins. | en_US |
dc.description.affiliation | Department of Microbiology, JJM Medical College, Davangere 577 004, Karnataka, India. mail@microrao.com | en_US |
dc.identifier.citation | Sridhar Rao PN, Basavarajappa KG, Krishna GL. Detection of extended spectrum beta-lactamase from clinical isolates in Davangere. Indian Journal of Pathology & Microbiology. 2008 Oct-Dec; 51(4): 497-9 | en_US |
dc.identifier.uri | https://imsear.searo.who.int/handle/123456789/73356 | |
dc.language.iso | eng | en_US |
dc.source.uri | https://www.ijpmonline.org | en_US |
dc.subject.mesh | Academic Medical Centers | en_US |
dc.subject.mesh | Anti-Bacterial Agents --pharmacology | en_US |
dc.subject.mesh | Cephalosporin Resistance | en_US |
dc.subject.mesh | Cephalosporins --pharmacology | en_US |
dc.subject.mesh | Enterobacteriaceae --classification | en_US |
dc.subject.mesh | Enterobacteriaceae Infections --microbiology | en_US |
dc.subject.mesh | Female | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Microbial Sensitivity Tests --methods | en_US |
dc.subject.mesh | beta-Lactam Resistance | en_US |
dc.subject.mesh | beta-Lactamases --biosynthesis | en_US |
dc.subject.mesh | beta-Lactams --pharmacology | en_US |
dc.title | Detection of extended spectrum beta-lactamase from clinical isolates in Davangere. | en_US |
dc.type | Journal Article | en_US |
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