Detection of extended spectrum beta-lactamase from clinical isolates in Davangere.

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dc.contributor.authorSridhar Rao, P Nen_US
dc.contributor.authorBasavarajappa, K Gen_US
dc.contributor.authorKrishna, G Leelaen_US
dc.date.accessioned2008-10-15en_US
dc.date.accessioned2009-05-29T11:17:38Z
dc.date.available2008-10-15en_US
dc.date.available2009-05-29T11:17:38Z
dc.date.issued2008-10-15en_US
dc.description.abstractExtended spectrum beta-lactamase enzymes (ESBLs) are enzymes that have the ability to hydrolyze oxyiminocephalosporins and infections by isolates producing them are often difficult to treat. A study to detect the presence of these enzymes in isolates was conducted by our hospital. A total of 207 non repetitive isolates were screened for resistance to any of five screening agents. Those with suspicious profiles were checked for ESBL production by double-disk approximation or a synergy test. The isolates were also subjected to a phenotypic confirmation test as recommended by CLSI (formerly NCCLS). Various cephalosporins-beta-lactamase inhibitor combinations were also tested. Of the 204 (98.5%) screen-positive isolates, only 126 (61.7%) were identified as ESBL producers. Of these, 26.1% of the isolates were positive by using the double-disk synergy test (DDST) method alone, 13.4% were positive using the method recommended by CLSI, and 60.3% of the isolates were positive by both the DDST and CLSI methods. We also report a high percentage of resistance to cefoxitin (96.8%) indicating changes in porins.en_US
dc.description.affiliationDepartment of Microbiology, JJM Medical College, Davangere 577 004, Karnataka, India. mail@microrao.comen_US
dc.identifier.citationSridhar Rao PN, Basavarajappa KG, Krishna GL. Detection of extended spectrum beta-lactamase from clinical isolates in Davangere. Indian Journal of Pathology & Microbiology. 2008 Oct-Dec; 51(4): 497-9en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/73356
dc.language.isoengen_US
dc.source.urihttps://www.ijpmonline.orgen_US
dc.subject.meshAcademic Medical Centersen_US
dc.subject.meshAnti-Bacterial Agents --pharmacologyen_US
dc.subject.meshCephalosporin Resistanceen_US
dc.subject.meshCephalosporins --pharmacologyen_US
dc.subject.meshEnterobacteriaceae --classificationen_US
dc.subject.meshEnterobacteriaceae Infections --microbiologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshMaleen_US
dc.subject.meshMicrobial Sensitivity Tests --methodsen_US
dc.subject.meshbeta-Lactam Resistanceen_US
dc.subject.meshbeta-Lactamases --biosynthesisen_US
dc.subject.meshbeta-Lactams --pharmacologyen_US
dc.titleDetection of extended spectrum beta-lactamase from clinical isolates in Davangere.en_US
dc.typeJournal Articleen_US
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