Revisiting the susceptibility testing of Mycobacterium tuberculosis to ethionamide in solid culture medium.

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dc.contributor.authorRajagopalan, Lakshmi
dc.contributor.authorRamachandran, Ranjani
dc.contributor.authorKumar, D Ravi
dc.contributor.authorSundar, A Syam
dc.contributor.authorRadhika, G
dc.contributor.authorRahman, Fathima
dc.contributor.authorSelvakumar, N
dc.contributor.authorKumar, Vanaja
dc.date.accessioned2016-08-03T05:37:32Z
dc.date.available2016-08-03T05:37:32Z
dc.date.issued2015-11
dc.description.abstractBackground & objectives: Increase in the isolation of drug resistant phenotypes of Mycobacterium tuberculosis necessitates accuracy in the testing methodology. Critical concentration defining resistance for ethionamide (ETO), needs re-evaluation in accordance with the current scenario. Thus, re-evaluation of conventional minimum inhibitory concentration (MIC) and proportion sensitivity testing (PST) methods for ETO was done to identify the ideal breakpoint concentration defining resistance. Methods: Isolates of M. tuberculosis (n=235) from new and treated patients were subjected to conventional MIC and PST methods for ETO following standard operating procedures. Results: With breakpoint concentration set at 114 and 156 μg/ml, an increase in specificity was observed whereas sensitivity was high with 80 μg/ml as breakpoint concentration. Errors due to false resistant and susceptible isolates were least at 80 μg/ml concentration. Interpretation & conclusions: Performance parameters at 80 μg/ml breakpoint concentration indicated significant association between PST and MIC methods.en_US
dc.identifier.citationRajagopalan Lakshmi, Ramachandran Ranjani, Kumar D Ravi, Sundar A Syam, Radhika G, Rahman Fathima, Selvakumar N, Kumar Vanaja. Revisiting the susceptibility testing of Mycobacterium tuberculosis to ethionamide in solid culture medium. Indian Journal of Medical Research. 2015 Nov; 142(5): 538-542.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/176341
dc.language.isoenen_US
dc.source.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4743339/en_US
dc.subjectDrug resistanceen_US
dc.subjectethionamideen_US
dc.subjectMIC methoden_US
dc.subjectMycobacterium tuberculosisen_US
dc.subjectproportion sensitivity testingen_US
dc.titleRevisiting the susceptibility testing of Mycobacterium tuberculosis to ethionamide in solid culture medium.en_US
dc.typeArticleen_US
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