Antibody classes & subclasses induced by mucosal immunization of mice with Streptococcus pyogenes M6 protein & oligodeoxynucleotides containing CpG motifs.

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dc.contributor.authorTeloni, Ren_US
dc.contributor.authorvon Hunolstein, Cen_US
dc.contributor.authorMariotti, Sen_US
dc.contributor.authorDonati, Sen_US
dc.contributor.authorOrefici, Gen_US
dc.contributor.authorNisini, Ren_US
dc.date.accessioned2004-05-03en_US
dc.date.accessioned2009-05-27T08:09:45Z
dc.date.available2004-05-03en_US
dc.date.available2009-05-27T08:09:45Z
dc.date.issued2004-05-03en_US
dc.description.abstractBACKGROUND & OBJECTIVES: Type-specific antibodies against M protein are critical for human protection as they enhance phagocytosis and are protective. An ideal vaccine for the protection against Streptococcus pyogenes would warrant mucosal immunity, but mucosally administered M-protein has been shown to be poorly immunogenic in animals. We used a recombinant M type 6 protein to immunize mice in the presence of synthetic oligodeoxynucleotides containing CpG motifs (immunostimulatory sequences: ISS) or cholera toxin (CT) to explore its possible usage in a mucosal vaccine. METHODS: Mice were immunized by intranasal (in) or intradermal (id) administration with four doses at weekly intervals of M6-protein (10 microg/mouse) with or without adjuvant (ISS, 10 microg/mouse or CT, 0,5 microg/mouse). M6 specific antibodies were measured by enzyme linked immunosorbent assay using class and subclass specific monoclonal antibodies. RESULTS: The use of ISS induced an impressive anti M-protein serum IgG response but when id administered was not detectable in the absence of adjuvant. When used in, M-protein in the presence of both ISS and CT induced anti M-protein IgA in the bronchoalveolar lavage, as well as specific IgG in the serum. IgG were able to react with serotype M6 strains of S. pyogenes. The level of antibodies obtained by immunizing mice in with M-protein and CT was higher in comparison to M-protein and ISS. The analysis of anti-M protein specific IgG subclasses showed high levels of IgG1, IgG2a and IgG2b, and low levels of IgG3 when ISS were used as adjuvant. Thus, in the presence of ISS, the ratio IgG2a/IgG1 and (IgG2a+IgG3)/IgG1 >1 indicated a type 1-like response obtained both in mucosally or systemically vaccinated mice. INTERPRETATION & CONCLUSION: Our study offers a reproducible model of anti-M protein vaccination that could be applied to test new antigenic formulations to induce an anti-group A Streptococcus (GAS) vaccination suitable for protection against the different diseases caused by this bacterium.en_US
dc.description.affiliationIstituto Superiore di Sanità, Rome, Italy.en_US
dc.identifier.citationTeloni R, von Hunolstein C, Mariotti S, Donati S, Orefici G, Nisini R. Antibody classes & subclasses induced by mucosal immunization of mice with Streptococcus pyogenes M6 protein & oligodeoxynucleotides containing CpG motifs. Indian Journal of Medical Research. 2004 May; 119 Suppl(): 126-30en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/23458
dc.language.isoengen_US
dc.source.urihttps://icmr.nic.in/ijmr/ijmr.htmen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntigens, Bacterial --administration & dosageen_US
dc.subject.meshBacterial Outer Membrane Proteins --administration & dosageen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshCarrier Proteins --administration & dosageen_US
dc.subject.meshCpG Islandsen_US
dc.subject.meshDNA Primersen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_US
dc.subject.meshMiceen_US
dc.subject.meshOligodeoxyribonucleotides --administration & dosageen_US
dc.subject.meshRecombinant Proteins --administration & dosageen_US
dc.titleAntibody classes & subclasses induced by mucosal immunization of mice with Streptococcus pyogenes M6 protein & oligodeoxynucleotides containing CpG motifs.en_US
dc.typeJournal Articleen_US
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