Effects of enteropathogenic bacteria & lactobacilli on chemokine secretion & Toll like receptor gene expression in two human colonic epithelial cell lines.

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dc.contributor.authorNandakumar, N S
dc.contributor.authorPugazhendhi, S
dc.contributor.authorRamakrishna, B S
dc.date.accessioned2011-12-15T03:43:46Z
dc.date.available2011-12-15T03:43:46Z
dc.date.issued2009-08
dc.description.abstractBackground & objectives: The intestinal epithelium is part of the innate immune system responding to contact with pathogenic or commensal bacteria. The objective of this study was to compare innate responses of intestinal epithelial cell lines to pathogenic bacteria and to lactobacilli. Methods: Two human intestinal epithelial cell lines, HT29 (enterocyte-like) and T84 (crypt-like), were exposed to pathogenic bacteria representative of non invasive (Vibrio cholerae O1 and O139), adherent (enterohaemorrhagic Escherichia coli, EHEC) or invasive (Salmonella Typhimurium and Shigella flexneri) phenotypes and to non pathogenic Lactobacillus rhamnosus GG or Lactobacillus plantarum. Interleukin-8 (IL-8) was measured in culture supernatant by ELISA, while mRNA from cells was subjected to quantitative reverse transcriptase PCR for several other chemokines (CXCL1, CCL5 and CXCL5) and for Toll-like receptors (TLR) 2, 4, 5 and 9. Results: V. cholerae, S. Typhimurium, S. flexneri and EHEC induced IL-8 secretion from epithelial cells into the medium. Salmonella, Shigella and EHEC, but not V. cholerae, significantly increased mRNA expression of CXCL1. None of the pathogens induced CCL5 or CXCL5. Salmonella and Vibrio significantly increased TLR4 expression, while Vibrio and EHEC decreased TLR5 expression. EHEC also decreased TLR9 expression. Lactobacilli attenuated the IL-8 response of the cell lines to V. cholerae, Salmonella, and EHEC but did not significantly change the IL-8 response to Shigella. Interpretation & conclusions: Distinct patterns of epithelial cell chemokine responses were induced by the bacterial pathogens studied and these were modulated by commensal lactobacilli. Alterations in TLR expression by these pathogens are likely to be important in pathogenesis.en_US
dc.identifier.citationNandakumar N S, Pugazhendhi S, Ramakrishna B S. Effects of enteropathogenic bacteria & lactobacilli on chemokine secretion & Toll like receptor gene expression in two human colonic epithelial cell lines. Indian Journal of Medical Research. 2009 Aug; 130(2): 170-178.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/135881
dc.language.isoenen_US
dc.source.urihttps://icmr.nic.in/ijmr/2009/august/0911.pdfen_US
dc.subjectChemokineen_US
dc.subjectenteropathogenic bacteriaen_US
dc.subjectinterleukin-8en_US
dc.subjectintestinal epithelial cellsen_US
dc.subjectLactobacillusen_US
dc.subjectToll-like receptoren_US
dc.subject.meshAnimals
dc.subject.meshCell Line
dc.subject.meshChemokines --immunology
dc.subject.meshChemokines --secretion
dc.subject.meshChild
dc.subject.meshColon --cytology
dc.subject.meshColon --microbiology
dc.subject.meshEnterohemorrhagic Escherichia coli --immunology
dc.subject.meshEpithelial Cells --cytology
dc.subject.meshEpithelial Cells --immunology
dc.subject.meshEpithelial Cells --microbiology
dc.subject.meshHumans
dc.subject.meshInterleukin-8 --immunology
dc.subject.meshInterleukin-8 --secretion
dc.subject.meshIntestinal Mucosa --cytology
dc.subject.meshIntestinal Mucosa --immunology
dc.subject.meshIntestinal Mucosa --microbiology
dc.subject.meshLactobacillus --immunology
dc.subject.meshSalmonella typhimurium --immunology
dc.subject.meshShigella flexneri --immunology
dc.subject.meshToll-Like Receptors --genetics
dc.subject.meshToll-Like Receptors --metabolism
dc.subject.meshVibrio cholerae O1 --immunology
dc.subject.meshVibrio cholerae O139 --immunology
dc.titleEffects of enteropathogenic bacteria & lactobacilli on chemokine secretion & Toll like receptor gene expression in two human colonic epithelial cell lines.en_US
dc.typeArticleen_US
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