Morphological Typing of Lymphomas with Immunohistochemistry.

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dc.contributor.authorBhardwaj, Aparna
dc.contributor.authorKishore, Sanjeev
dc.contributor.authorKusum, Anuradha
dc.date.accessioned2015-07-08T06:11:32Z
dc.date.available2015-07-08T06:11:32Z
dc.date.issued2015-04
dc.description.abstractLymphoid malignancies (LM) are a heterogeneous group of disorders that are broadly divided into Hodgkin disease (HD) and Non-Hodgkin Lymphoma (NHL). Diagnosing lymphoid malignancies based on morphology in conjunction with immunohistochemistry (IHC) forms the basis of WHO classification and this has prognostic implications.With this background this study was designed thus including all the lymphoid malignancies both NHL and HD. Materials and Methods: This study was conducted at a tertiary centre in Uttarakhand and included a total of 116 cases of lymphoid malignancies. Of these 41 cases were of Hodgkin disease and 75 cases were of NHL. These cases were initially diagnosed on morphology employing Hematoxylin and Eosin (H&E) and special stains like Reticulin. Subsequently, a preliminary panel of monoclonal antibodies using CD3, CD15, CD20, CD30, and CD45 were employed. All the cases were then classified using WHO classification. Results: HD- Of the 41 cases of Hodgkin’s disease the commonest subtype was Nodular Sclerosis seen in 26 cases (48.78%). Reed Sternberg in reactive milieu is diagnostic of Hodgkin disease. In all cases except one Reed Sternberg cells exhibited positivity for both CD15 and CD30. NHL – Of the 75 cases of NHL an initial classification based on morphology was done. All the cases were classified according to International Working Formulation initially. Subsequently, IHC was employed using CD3, CD15, CD20 and CD45. The disease was then classified according to WHO classification and broadly divided into B or T cell types. B cell expression was seen in 60 cases (80%) and T cell expression in 15 cases (20%). The commonest B cell subtype was Diffuse Large B cell Lymphoma (26.4%).en_US
dc.identifier.citationBhardwaj Aparna, Kishore Sanjeev, Kusum Anuradha. Morphological Typing of Lymphomas with Immunohistochemistry. Indian Medical Gazette. 2015 Apr; 149 (4): 127-133.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/159279
dc.language.isoenen_US
dc.source.urihttps://medind.nic.in/ice/t15/i4/icet15i4p127.pdfen_US
dc.subjectlymphoid malignanciesen_US
dc.subjecthodgkin diseaseen_US
dc.subjectnon- hodgkin lymphomaen_US
dc.subjectimmunohistochemistryen_US
dc.subject.meshAdolescent
dc.subject.meshAdult
dc.subject.meshChild
dc.subject.meshFemale
dc.subject.meshHodgkin Disease --immunology
dc.subject.meshHumans
dc.subject.meshImmunohistochemistry --methods
dc.subject.meshLymph Nodes --immunology
dc.subject.meshLymphocytes --immunology
dc.subject.meshLymphoma --immunology
dc.subject.meshLymphoma, Non-Hodgkin --immunology
dc.subject.meshMale
dc.subject.meshMiddle Aged
dc.subject.meshYoung Adult
dc.titleMorphological Typing of Lymphomas with Immunohistochemistry.en_US
dc.typeArticleen_US
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