Morphological Typing of Lymphomas with Immunohistochemistry.
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Date
2015-04
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Abstract
Lymphoid malignancies (LM) are a heterogeneous group
of disorders that are broadly divided into Hodgkin disease
(HD) and Non-Hodgkin Lymphoma (NHL). Diagnosing
lymphoid malignancies based on morphology in conjunction
with immunohistochemistry (IHC) forms the basis of WHO
classification and this has prognostic implications.With this
background this study was designed thus including all the
lymphoid malignancies both NHL and HD. Materials and
Methods: This study was conducted at a tertiary centre in
Uttarakhand and included a total of 116 cases of lymphoid
malignancies. Of these 41 cases were of Hodgkin disease
and 75 cases were of NHL. These cases were initially
diagnosed on morphology employing Hematoxylin and Eosin
(H&E) and special stains like Reticulin. Subsequently, a
preliminary panel of monoclonal antibodies using CD3,
CD15, CD20, CD30, and CD45 were employed. All the
cases were then classified using WHO classification.
Results: HD- Of the 41 cases of Hodgkin’s disease the
commonest subtype was Nodular Sclerosis seen in 26 cases
(48.78%). Reed Sternberg in reactive milieu is diagnostic
of Hodgkin disease. In all cases except one Reed Sternberg
cells exhibited positivity for both CD15 and CD30. NHL –
Of the 75 cases of NHL an initial classification based on
morphology was done. All the cases were classified
according to International Working Formulation initially. Subsequently, IHC was employed using CD3, CD15, CD20
and CD45. The disease was then classified according to
WHO classification and broadly divided into B or T cell
types. B cell expression was seen in 60 cases (80%) and T
cell expression in 15 cases (20%). The commonest B cell
subtype was Diffuse Large B cell Lymphoma (26.4%).
Description
Keywords
lymphoid malignancies, hodgkin disease, non- hodgkin lymphoma, immunohistochemistry
Citation
Bhardwaj Aparna, Kishore Sanjeev, Kusum Anuradha. Morphological Typing of Lymphomas with Immunohistochemistry. Indian Medical Gazette. 2015 Apr; 149 (4): 127-133.