Aberrant signal transduction in Indian triple-negative breast cancer patients

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dc.contributor.authorBhaskara, Vasantha Kumaren_US
dc.contributor.authorJayaram, Chaitraen_US
dc.contributor.authorPriyanga, Men_US
dc.contributor.authorNayaka, N H Thilaken_US
dc.contributor.authorShivakumara, Aen_US
dc.contributor.authorAmaresh, Nagarathnaen_US
dc.contributor.authorMysorekar, Vijaya Ven_US
dc.date.accessioned2020-11-18T10:07:27Z
dc.date.available2020-11-18T10:07:27Z
dc.date.issued2019-10
dc.description.abstractAim of Study: The aim of this study is to correlate the prominin-1 or CD133 association with functional pathway markers of cancer stemness in Indian triple-negative breast cancer (TNBC) patient samples. Materials and Methods: TNBC samples were confirmed for the absence of hormone receptors (estrogen receptor–ER/progesterone receptor) and human epidermal growth factor receptor-2 or proto-oncogene neu or erbB2 or CD340 by immunohistochemical analysis. Formalin-fixed paraffin-embedded samples of patients were used to collect the total RNA. Then, one-step reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the cancer stemness-related transcript levels in the different samples. The RT-PCR products were analyzed semi-quantitatively on agarose gels. The band intensities of respective samples for different transcripts were analyzed by densitometry. Results: TNBC-confirmed samples had shown increased levels of CD133 transcript than control tissues. Further, elevated CD133 transcripts are correlated with higher transcript levels of NOTCH1/FZD7/transforming growth factor-beta receptor Type III R/patched-1 pathway mediators. Conclusions: This work has clearly indicated that there is a correlation between CD133 and functional pathways that control cancer stem cells in TNBC. These observations may indicate the possible association between cancer stemness and TNBC malignancyen_US
dc.identifier.affiliationsDepartment of Biochemistry, RCASC, Bengaluru, Karnataka, Indiaen_US
dc.identifier.affiliationsDepartment of Biochemistry, RCASC, Bengaluru, Karnataka, Indiaen_US
dc.identifier.affiliationsDepartment of Biochemistry, RCASC, Bengaluru, Karnataka, Indiaen_US
dc.identifier.affiliationsDepartment of Biochemistry, RCASC, Bengaluru, Karnataka, Indiaen_US
dc.identifier.affiliationsDepartment of Biochemistry, RCASC, Bengaluru, Karnataka, Indiaen_US
dc.identifier.affiliationsDepartment of Biochemistry, RCASC, Bengaluru, Karnataka, Indiaen_US
dc.identifier.affiliationsDepartment of Pathology, RMCH, Bengaluru, Karnataka, Indiaen_US
dc.identifier.citationBhaskara Vasantha Kumar, Jayaram Chaitra, Priyanga M, Nayaka N H Thilak, Shivakumara A, Amaresh Nagarathna, Mysorekar Vijaya V. Aberrant signal transduction in Indian triple-negative breast cancer patients. Journal of Cancer Research and Therapeutics. 2019 Oct; 15(5): 1162-1166en_US
dc.identifier.issn0973-1482
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/213495
dc.languageenen_US
dc.publisherWolters Kluwer India Pvt. Ltd.en_US
dc.relation.issuenumber5en_US
dc.relation.volume15en_US
dc.source.urihttps://dx.doi.org//10.4103/jcrt.JCRT_803_16en_US
dc.subjectCancer stem cellsen_US
dc.subjectFZD7en_US
dc.subjectNOTCH1en_US
dc.subjectpatched‑1en_US
dc.subjectsignal transductionen_US
dc.subjecttransforming growth factor‑beta receptor Type III Ren_US
dc.subjecttriple‑negative breast canceren_US
dc.titleAberrant signal transduction in Indian triple-negative breast cancer patientsen_US
dc.typeJournal Articleen_US
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