Aberrant signal transduction in Indian triple-negative breast cancer patients

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Ijcrt2019v15n5p1162.pdf (1.24 MB)
Date
2019-10
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Wolters Kluwer India Pvt. Ltd.
Abstract
Aim of Study: The aim of this study is to correlate the prominin-1 or CD133 association with functional pathway markers of cancer stemness in Indian triple-negative breast cancer (TNBC) patient samples. Materials and Methods: TNBC samples were confirmed for the absence of hormone receptors (estrogen receptor–ER/progesterone receptor) and human epidermal growth factor receptor-2 or proto-oncogene neu or erbB2 or CD340 by immunohistochemical analysis. Formalin-fixed paraffin-embedded samples of patients were used to collect the total RNA. Then, one-step reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the cancer stemness-related transcript levels in the different samples. The RT-PCR products were analyzed semi-quantitatively on agarose gels. The band intensities of respective samples for different transcripts were analyzed by densitometry. Results: TNBC-confirmed samples had shown increased levels of CD133 transcript than control tissues. Further, elevated CD133 transcripts are correlated with higher transcript levels of NOTCH1/FZD7/transforming growth factor-beta receptor Type III R/patched-1 pathway mediators. Conclusions: This work has clearly indicated that there is a correlation between CD133 and functional pathways that control cancer stem cells in TNBC. These observations may indicate the possible association between cancer stemness and TNBC malignancy
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Keywords
Cancer stem cells, FZD7, NOTCH1, patched‑1, signal transduction, transforming growth factor‑beta receptor Type III R, triple‑negative breast cancer
Citation
Bhaskara Vasantha Kumar, Jayaram Chaitra, Priyanga M, Nayaka N H Thilak, Shivakumara A, Amaresh Nagarathna, Mysorekar Vijaya V. Aberrant signal transduction in Indian triple-negative breast cancer patients. Journal of Cancer Research and Therapeutics. 2019 Oct; 15(5): 1162-1166
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https://imsear.searo.who.int/handle/123456789/213495
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Journal of Cancer Research and Therapeutics