Identification of novel polymorphism in buffalo stanniocalcin-1 gene and its expression analysis in mammary gland under different stages of lactation

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dc.contributor.authorMishra, S. K.en_US
dc.contributor.authorDUBEY, P. K.en_US
dc.contributor.authorGOYAL, S.en_US
dc.contributor.authorSINGH, S.en_US
dc.contributor.authorNIRANJAN, S.K.en_US
dc.contributor.authorVOHRA, V.en_US
dc.contributor.authorMUKESH, M.en_US
dc.contributor.authorKATARIA, R. S.en_US
dc.date.accessioned2020-11-18T10:20:51Z
dc.date.available2020-11-18T10:20:51Z
dc.date.issued2019-04
dc.description.abstractStanniocalcin-1 (STC1) is secreted by the variety of tissues having a major role in the regulation of calcium ions in the involuting mammary gland. The present work aims to sequence and structural characterization as well as expression profiling of STC1 gene in buffalo. Polymorphism identified in the 3-untranslated region (UTR) was analysed by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) genotyping in riverine and swamp buffaloes. Expression profiling of STC1 was performed in different lactation stages of mammary gland and peripheral blood mononuclear cells to study the impact of 3'-UTR polymorphism on its expression. Different polymorphic sites were detected in the entire coding and noncoding regions of riverine and swamp buffaloes, including two INDELs. An identified polymorphic nucleotide locus A324G, having target sites for two miRNAs, namely bta-miR-2382 and bta-miR-1343, reported in cattle, was genotyped by PCR-RFLP to reveal variable allelic distribution among swamp and riverine buffaloes. Gene expression profiling across buffalo mammary tissues representing different lactation stages showed maximum expression of the STC1 gene in the involuting mammary gland. Ruminants’ specific genetic variation has been observed in STC1 and its implication in buffalo mammary gland involution as well as coregulation of gene expression throughmiRNA binding in the 3'-UTR is suggested.en_US
dc.identifier.affiliationsICAR-National Bureau of Animal Genetic Resources, Karnal 132 001, Indiaen_US
dc.identifier.affiliationsLewis Ketz School of Medicine, Temple University, Philadelphia, PA, USAen_US
dc.identifier.citationMishra S. K., Dubey P. K., Goyal S., Singh S., Niranjan S.K., Vohra V., Mukesh M., Kataria R. S.. Identification of novel polymorphism in buffalo stanniocalcin-1 gene and its expression analysis in mammary gland under different stages of lactation. Journal of Genetics. 2019 Apr; 98: 1-8en_US
dc.identifier.issn0022-1333
dc.identifier.issn0973-7731
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/215459
dc.languageenen_US
dc.publisherIndian Academy of Sciencesen_US
dc.relation.volume98en_US
dc.source.urihttps://www.ias.ac.in/article/fulltext/jgen/098/0038en_US
dc.subjectSTC1 geneen_US
dc.subjectpolymorphismen_US
dc.subject3-untranslated regionen_US
dc.subjectmicro-RNA target sitesen_US
dc.subjectexpression analysisen_US
dc.subjectBubalus bubalisen_US
dc.titleIdentification of novel polymorphism in buffalo stanniocalcin-1 gene and its expression analysis in mammary gland under different stages of lactationen_US
dc.typeJournal Articleen_US
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