Comparison between DOT EIA IgM and Widal Test as early Diagnosis of Typhoid Fever.

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dc.contributor.authorBegum, Zen_US
dc.contributor.authorHossain, M Aen_US
dc.contributor.authorMusa, A Ken_US
dc.contributor.authorShamsuzzaman, A Ken_US
dc.contributor.authorMahmud, M Cen_US
dc.contributor.authorAhsan, M Men_US
dc.contributor.authorSumona, A Aen_US
dc.contributor.authorAhmed, Sen_US
dc.contributor.authorJahan, N Aen_US
dc.contributor.authorAlam, Men_US
dc.contributor.authorBegum, Aen_US
dc.date.accessioned2009-01-03en_US
dc.date.accessioned2009-05-27T03:53:02Z
dc.date.available2009-01-03en_US
dc.date.available2009-05-27T03:53:02Z
dc.date.issued2009-01-03en_US
dc.descriptionMymensingh Medical Journal.en_US
dc.description.abstractA recently developed DOT enzyme immunoassay known as "Typhidot" for detecting IgM antibody against 50 KDa OMP antigen of Salmonella typhi, was evaluated on 100 clinically suspected typhoid fever cases and 40 age-sex matched controls, in the Department of Microbiology, Mymensingh Medical College during, the period from June 2006 to July 2007. Blood culture, Widal test, and DOT EIA for IgM test were performed in all patients. Among 100 clinically suspected typhoid fever cases, 35 were subsequently confirmed on the basis of positive blood culture for S. typhi and/or significant rising titre of Widal test. The DOT EIA IgM test could produce results within 1 hour. The result of the DOT EIA IgM test showed a good diagnostic value for typhoid fever. The sensitivity, specificity, positive and negative predictive value of the test was found as 91.42%, 90.00%, 88.88% and 92.30% respectively. On the other hand corresponding values for Widal test were of 42.85%, 85.00%, 71.42% and 62.96% respectively. Thus, The DOT EIA IgM seems to be a practical alternative to Widal test for early diagnosis of typhoid fever.en_US
dc.description.affiliationDr Zohra Begum, Assistant Professor, Department of Microbiology, CBMCB, Mymensingh, Bangladesh.en_US
dc.identifier.citationBegum Z, Hossain MA, Musa AK, Shamsuzzaman AK, Mahmud MC, Ahsan MM, Sumona AA, Ahmed S, Jahan NA, Alam M, Begum A. Comparison between DOT EIA IgM and Widal Test as early Diagnosis of Typhoid Fever. Mymensingh Medical Journal. 2009 Jan; 18(1): 13-7en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/1241
dc.language.isoengen_US
dc.titleComparison between DOT EIA IgM and Widal Test as early Diagnosis of Typhoid Fever.en_US
dc.typeJournal Articleen_US
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