Nitric oxide and caspase 3 mediated cytokine induced apoptosis in acute leukemia.

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dc.contributor.authorSiripin, Darin
dc.contributor.authorFucharoen, Suthat
dc.contributor.authorTanyong, Dalina I
dc.date.accessioned2012-01-11T10:33:01Z
dc.date.available2012-01-11T10:33:01Z
dc.date.issued2010-03
dc.description.abstractBackground: Leukemia is characterized by the uncontrolled accumulation of white blood cells. Recently, cytokines have been used in immunotherapy, which is a new strategy for leukemia treatment. Objective: To investigate the effect of cytokines on induction of apoptosis in acute leukemic cell lines; HL-60, MV4-11, K-562 and Molt-4 and in addition, to study the involvement of nitric oxide (NO) in apoptotic pathways. Methods: Leukemic cell lines were incubated with cytokines; interleukin-1β, tumor necrosis factor-a, and interferon-γ in various concentrations and for variable periods of time. The percent apoptosis and caspase 3 activation were examined by flow cytometry. Moreover, NO production and inducible nitric oxide synthase (iNOS) mRNA were measured by using Griess method and Real-time PCR, respectively. Results: Cytokines caused a time and dose-dependent induction of apoptosis in leukemic cell lines. The highest cell apoptosis was found in K-562 treated with 40 U/ml interferon-γ for 48 hours; this correlated with the result of cell growth inhibition and caspase 3 activation. NO and iNOS mRNA were increased in cytokines treated cells. Moreover, apoptosis was reduced by SMT, an iNOS inhibitor, which confirms the possible involvement of NO in the apoptotic pathway. Conclusion: Cytokines especially interferon-g induced apoptosis in acute leukemia via NO and caspase 3 pathway.en_US
dc.identifier.citationSiripin Darin, Fucharoen Suthat, Tanyong Dalina I. Nitric oxide and caspase 3 mediated cytokine induced apoptosis in acute leukemia. Asian Pacific Journal of Allergy and Immunology, 2011 Mar; 29(1): 102-111.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/136403
dc.language.isoenen_US
dc.source.urihttps://apjai.digitaljournals.org/index.php/apjai/article/viewFile/388/355en_US
dc.subjectLeukemiaen_US
dc.subjectapoptosisen_US
dc.subjectcytokineen_US
dc.subjectnitric oxideen_US
dc.subjectcaspaseen_US
dc.titleNitric oxide and caspase 3 mediated cytokine induced apoptosis in acute leukemia.en_US
dc.typeArticleen_US
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