Use of Palm Oil Mill Effluent as Medium for Cultivation of Chlorella sorokiniana.
Loading...
Date
2014-03
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Aims: Palm oil mill effluent (POME) erodes the principal biophysical characteristic of both
soil and water when discharged untreated but could be exploited as medium for
microalgae cultivation due to its vast mineral contents.
Place and Duration of study: POME samples were collected from a local palm oil
processing mill at Nsukka, Enugu State, Nigeria. A part of the study was done at the
Graduate School of Life and Environmental Sciences, University of Tsukuba, Japan while
the rest at the University of Nigeria, Nsukka between March and September, 2012.
Methodology: Chlorella sorokiniana C212 was grown in several Batches (A-D) of POME
supplemented with urea (60 mg/L) before subjecting to different sterilization protocols.
Cultivation was conducted in shaker flasks at 150 rpm, 1 vvm, 3000 lux and pH 7.0±0.2.
Results: The filter sterilized Batch (B) promoted the highest (1070±30 mg/L) dry cell
weight (DCW), lipid (156±12 mg/g-cell) and chlorophyll (1.59±0.11 mg/g-cell) contents
while chemical oxygen demand (COD) decreased by 45±08%. The autoclaved medium (Batch A) gave the least DCW (310±20 mg/L), lipid production (40±05 mg/g-cell) and
chlorophyll content (0.58±0.02 mg/g-cell) while COD reduced by 20±04%. The highest
COD decrease (70±05%) was achieved in the unsterilized Batch (D). Batch B was most
positively affected by dilution because at 75% concentration, DCW increased to 1360±30
mg/L, lipid contents to 174±10 mg/g-cell, chlorophyll to 1.87±0.14 mg/g-cell the while
COD declined by 63±03%.
Conclusions: POME has potential for use in microalgae cultivation with significant saving
in treatment costs.
Description
Keywords
Palm oil mill effluent, microalgae, cultivation, chlorella sorokiniana, sterilization, chlorophyll, chemical oxygen demand, dilution
Citation
Nwuche Charles Ogugua, Ekpo Doris Chidimma, Eze Chijioke Nwoye, Aoyagi Hideki, Ogbonna James Chukwuma. Use of Palm Oil Mill Effluent as Medium for Cultivation of Chlorella sorokiniana. British Biotechnology Journal. 2014 Mar; 4(3): 305-316.