Cytological intracellular glycogen evaluation using PAS and PAS-D stains to correlate plasma glucose in diabetics

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dc.contributor.authorRavikumar, S Shamalaen_US
dc.contributor.authorMenaka, TRen_US
dc.contributor.authorVasupradha, Gen_US
dc.contributor.authorDhivya, Ken_US
dc.contributor.authorDinakaran, Jen_US
dc.contributor.authorSaranya, Ven_US
dc.date.accessioned2020-01-02T06:46:59Z
dc.date.available2020-01-02T06:46:59Z
dc.date.issued2019-09
dc.description.abstractBackground: Oral cytology studies have claimed that cytoplasmic Periodic Acid Schiff (PAS) positivity in type-2 diabetics is due to glycogen content. But, it can also be due to mucin and glycoconjugates. Aim: 1. To confirm that cytoplasmic PAS positivity in type-2 diabetics is due to glycogen using diastase. 2. To know the effect of diabetes by determining the number of glycogen-containing cells in the smear. 3. To assess the impact of duration of diabetes based on PAS staining of cells. 4. To correlate between random blood glucose level and the number of PAS-positive cells. Materials and Methods: Study population comprised 45 individuals with 30 type-2 diabetics as case group (Group I < 5 years duration; Group II > 5 years duration) and 15 healthy volunteers (age and gender-matched) as control. For all subjects, random blood glucose was estimated and two cytosmears were obtained. The smears were stained with PAS and PAS-diastase stains (PAS-D). Staining intensity was documented as score 1 (mild-to-moderate) and score 2 (moderate-to-intense) and data obtained were statistically analyzed in SPSS version 16.0. Results: Mann-Whitney U test revealed that in diabetics cytoplasmic PAS positivity is because of glycogen (P < 0.05). There is an increase in the number of glycogen-containing cells (P < 0.05) in diabetics. The duration of diabetes had less impact on intracellular glycogen accumulation (P > 0.05). Spearman's correlation test revealed no significant correlation (P > 0.05) between random blood glucose and a number of PAS-positive cells. Conclusion: PAS positivity is because of intracellular glycogen accumulation in type-2 diabetics. It can convey the glycaemic status of an individual in the recent past, thus a beneficial role in screening and therapeutic monitoring.en_US
dc.identifier.affiliationsDepartment of Oral and Maxillofacial Pathology, Adhiparasakthi Dental College and Hospital, Melmaruvathur, Tamil Nadu, Indiaen_US
dc.identifier.citationRavikumar S Shamala, Menaka TR, Vasupradha G, Dhivya K, Dinakaran J, Saranya V. Cytological intracellular glycogen evaluation using PAS and PAS-D stains to correlate plasma glucose in diabetics. Indian Journal of Dental Research. 2019 Sep; 30(5): 703-707en_US
dc.identifier.issn0970-9290
dc.identifier.issn1998-3603
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/192302
dc.languageenen_US
dc.publisherIndian Society for Dental Researchen_US
dc.relation.issuenumber5en_US
dc.relation.volume30en_US
dc.source.urihttps://dx.doi.org/10.4103/ijdr.IJDR_815_18en_US
dc.subjectCytologyen_US
dc.subjectdiabetesen_US
dc.subjectdiastaseen_US
dc.subjectglucose levelsen_US
dc.subjectglycogenen_US
dc.subjectperiodic acid Schiffen_US
dc.titleCytological intracellular glycogen evaluation using PAS and PAS-D stains to correlate plasma glucose in diabeticsen_US
dc.typeJournal Articleen_US
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