Efficient in vitro plant regeneration via leaf base segments of indica rice (Oryza sativa L.).

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dc.contributor.authorRamesh, Men_US
dc.contributor.authorMurugiah, Ven_US
dc.contributor.authorGupta, Aditya Ken_US
dc.date.accessioned2009-05-28T12:38:15Z
dc.date.available2009-05-28T12:38:15Z
dc.date.issued2009-01-26en_US
dc.description.abstractA reliable and reproducible protocol has been developed for high frequency plant regeneration from 4-5 mm long leaf base segments of 4 days old in vitro germinated seedlings of indica rice (Oryza sativa) cultivar Rasi. The effect of age of seedlings, position of leaf base segments and optimum concentration of 2,4-D on callus induction frequency was investigated with a future aim to use leaf bases for biolistic and Agrobacterium-mediated transformation experiments. Friable, nodular and white to pale yellow embryogenic callus cultures (206 mg fresh weight /explant) were obtained from the first basal segments of rice seedlings on Linsmaier and Skoog (LS) medium supplemented with 2,4-D (11.3 microM) and 3.0 microM thiamine-HCL. Plant regeneration was achieved after the transfer of 54 days old embryogenic callus cultures to Murashige and Skoog (MS) medium supplemented with BAP (2.2 microM) and NAA (0.27 microM). In vitro regenerated plants with multiple shoots and roots transferred to sterile soil in growth chamber and maintained in greenhouse exhibited normal growth and were phenotypically similar to plants germinated from seeds.en_US
dc.description.affiliationDepartment of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai 625 021, India. ramesh_bioteck@yahoo.comen_US
dc.identifier.citationRamesh M, Murugiah V, Gupta AK. Efficient in vitro plant regeneration via leaf base segments of indica rice (Oryza sativa L.). Indian Journal of Experimental Biology. 2009 Jan; 47(1): 68-74en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/58351
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.source.urihttps://nopr.niscair.res.in/bitstream/123456789/2898/1/IJEB%2047%281%29%2068-74.pdfen_US
dc.subject.mesh2,4-Dichlorophenoxyacetic Acid --pharmacology
dc.subject.meshCulture Media
dc.subject.meshEmbryonic Development --drug effects
dc.subject.meshOryza sativa --drug effects
dc.subject.meshOryza sativa --embryology
dc.subject.meshOryza sativa --physiology
dc.subject.meshPlant Leaves --drug effects
dc.subject.meshPlant Leaves --growth & development
dc.subject.meshPlant Leaves --physiology
dc.subject.meshRegeneration --drug effects
dc.subject.meshTime Factors
dc.titleEfficient in vitro plant regeneration via leaf base segments of indica rice (Oryza sativa L.).en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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