Role of light and immunofluorescence microscopy to differentiate primary and secondary membranous nephropathy

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dc.contributor.authorBasu, Ken_US
dc.contributor.authorSengupta, Men_US
dc.contributor.authorMukherjee, Sen_US
dc.contributor.authorKarmakar, Sen_US
dc.contributor.authorRoychowdhury, Aen_US
dc.contributor.authorBandopadhyay, M.en_US
dc.date.accessioned2023-08-10T07:32:57Z
dc.date.available2023-08-10T07:32:57Z
dc.date.issued2022-12
dc.description.abstractContext: Membranous nephropathy (MN) causes nephrotic syndrome, mostly primary but may be associated with SLE, infections, cancer, or drug. Aims: To estimate clinical, serological, light microscopic, and direct immunofluorescence (DIF) findings to differentiate primary and secondary MN. Settings and Design: Prospective, cross-sectional, single-center study in a tertiary care hospital. Methods and Material: Total 51 cases from September 2019 to February 2020. Laboratory Data: Blood glucose, urine analysis, urea, creatinine, albumin, cholesterol, HBsAg, Anti HCV, ASO, ANA, MPO ANCA, PR3 ANCA, dsDNA, PLA2R, C3, and C4. Clinical parameters: age, sex, BP, skin lesions, arthralgia, edema, obesity. Renal biopsies examined with H and E, PAS, silver methanamine, MT stains. DIF done with IgG, IgM, IgA, C3c, C1q, kappa, and lambda. Statistical Analysis Used: Statistical software (Graph Pad PRISM 6) and Chi-square test). Results: Among 51 cases, 25 are primary and 26 are secondary MN with 22 being lupus nephritis, with 2 being post-infectious and the remaining 2 being proliferative glomerulonephritis with monoclonal immunoglobulin deposition (PGNMIDD) with kappa chain restriction. Mean age was 37 ± 12.18 and 30.69 ± 13.92 years for primary and secondary MN, respectively. Significant male preponderance in primary MN. Serum C4 significantly low in secondary MN (15.34 ± 9.59). Microscopic hematuria present in secondary MN. Mesangial and endocapillary hypercellularity are significant in secondary MN. IgG and kappa are significantly intense in primary whereas IgA, C3c, and C1q are significantly intense in secondary MN. Conclusions: Reliable differentiation between primary and secondary MN has important therapeutic implications.en_US
dc.identifier.affiliationsDepartment of Pathology, Institute of Postgraduate Medical Education and Research, Health University, Kolkata, West Bengal, Indiaen_US
dc.identifier.affiliationsDepartment of Nephrology, Institute of Postgraduate Medical Education and Research, Health University, Kolkata, West Bengal, Indiaen_US
dc.identifier.citationBasu K, Sengupta M, Mukherjee S, Karmakar S, Roychowdhury A, Bandopadhyay M.. Role of light and immunofluorescence microscopy to differentiate primary and secondary membranous nephropathy. Indian Journal of Pathology & Microbiology. 2022 Dec; 65(4): 821-827en_US
dc.identifier.issn0377-4929
dc.identifier.issn0974-5130
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/223351
dc.languageenen_US
dc.publisherWolters Kluwer - Medknowen_US
dc.relation.issuenumber4en_US
dc.relation.volume65en_US
dc.source.urihttps://doi.org/10.4103/ijpm.ijpm_22_21en_US
dc.subjectImmunofluorescenceen_US
dc.subjectlupus nephritisen_US
dc.subjectmembranous nephropathyen_US
dc.subjectsecondaryen_US
dc.titleRole of light and immunofluorescence microscopy to differentiate primary and secondary membranous nephropathyen_US
dc.typeJournal Articleen_US
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