Phenol ammonium sulphate basic fuchsin staining of sputum in pot for the detection of acid-fast bacilli.

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dc.contributor.authorSelvakumar, Nen_US
dc.contributor.authorPremkumar, Michelen_US
dc.contributor.authorKumar, Vanajaen_US
dc.contributor.authorGopi, P Gen_US
dc.contributor.authorSivagamasundari, Sen_US
dc.contributor.authorPrabhakaran, Een_US
dc.contributor.authorNarayanan, P Ren_US
dc.date.accessioned2008-08-13en_US
dc.date.accessioned2009-05-27T08:09:24Z
dc.date.available2008-08-13en_US
dc.date.available2009-05-27T08:09:24Z
dc.date.issued2008-08-13en_US
dc.description.abstractBACKGROUND & OBJECTIVES: Improper practices of making direct smears of sputum for detection of acid-fast bacilli (AFB) and of disposing sputum cups are hazardous. The present study was undertaken with the objective to stain sputum samples in their containers by 'phenol (10%) ammonium sulphate (4%) basic fuchsin (2%) solution' and to decolourize and counterstain their smears for detection of AFB- (henceforth called pot method) and to compare the smear results of pot method with the standard Ziehl-Neelsen (ZN) method. METHODS: A total of 575 selected sputum samples from pulmonary tuberculosis patients were stained by the standard ZN and pot methods and the proportions of AFB positive smears were compared. RESULTS: Of the 575 samples, 126 were AFB positive for both the staining methods and the difference was not statistically significant. Pot method missed 9 ZN positive smears (8 scanty and one 1+) and ZN method missed 9 pot positive smears (9 scanty) and the difference was not significant. High grade smears (3+) were seen more in pot method (42) than in ZN method (25) and the difference was significant. INTERPRETATION & CONCLUSION: Our findings showed that pot method was comparable to standard ZN method and had many advantages. Pot method can be explored further for the detection of AFB in sputum samples obtained from pulmonary tuberculosis suspects.en_US
dc.description.affiliationTuberculosis Research Centre (ICMR), Chetput, Chennai, India. selvakumar.nagamiah@gmail.comen_US
dc.identifier.citationSelvakumar N, Premkumar M, Kumar V, Gopi PG, Sivagamasundari S, Prabhakaran E, Narayanan PR. Phenol ammonium sulphate basic fuchsin staining of sputum in pot for the detection of acid-fast bacilli. Indian Journal of Medical Research. 2008 Aug; 128(2): 194-7en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/23436
dc.language.isoengen_US
dc.source.urihttps://icmr.nic.in/ijmr/ijmr.htmen_US
dc.subject.meshHumansen_US
dc.subject.meshMycobacterium --isolation & purificationen_US
dc.subject.meshRosaniline Dyesen_US
dc.subject.meshSpecimen Handling --methodsen_US
dc.subject.meshSputum --microbiologyen_US
dc.subject.meshStaining and Labeling --methodsen_US
dc.subject.meshTuberculosis, Pulmonary --diagnosisen_US
dc.titlePhenol ammonium sulphate basic fuchsin staining of sputum in pot for the detection of acid-fast bacilli.en_US
dc.typeComparative Studyen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
dc.typeResearch Support, U.S. Gov't, Non-P.H.S.en_US
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