A novel method of staining acid-fast bacilli in sputum containers.

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dc.contributor.authorSelvakumar, Nen_US
dc.contributor.authorRavikumar, Den_US
dc.contributor.authorSivagamasundari, Sen_US
dc.contributor.authorGopi, P Gen_US
dc.contributor.authorNarayanan, P Ren_US
dc.date.accessioned2006-06-04en_US
dc.date.accessioned2009-05-27T07:58:37Z
dc.date.available2006-06-04en_US
dc.date.available2009-05-27T07:58:37Z
dc.date.issued2006-06-04en_US
dc.description.abstractBACKGROUND & OBJECTIVES: Making centrifuged deposit smears from sputum to detect acid-fast bacilli (AFB) is considered hazardous. We carried out this study to stain the centrifuged deposits with carbol-fuchsin in sputum containers and to decolourize and counterstain their smears made on glass slides. METHODS: The centrifuged deposits of 180 sputum samples from pulmonary tuberculosis patients were used for making smears (initial deposit smears) and staining by Ziehl-Neelsen (ZN) method for the detection of AFB. Each of the sputum deposit was then treated with one ml of 1 per cent carbol-fuchsin and a smear made between 2 to 3 h was then decolourized and counterstained by the same procedures followed in ZN method (2 h stained deposit smear). The coded initial deposit smears and the corresponding 2 h stained deposit smears were read by the same readers and the results compared. RESULTS: One hundred and fifty (70 positive and 80 negative) 2 h stained deposit smears were compared with initial deposit smears and the difference was not statistically significant. INTERPRETATION & CONCLUSION: Centrifuged deposits of sputum in sputum containers can be stained by carbol-fuchsin within 2-3 h and their smears made subsequently on glass slides can then be decolourized and counterstained by the procedures followed in ZN method for detection of AFB by light microscopy.en_US
dc.description.affiliationTuberculosis Research Centre (ICMR) Chennai, India. selvakumar.nagamiah@gmail.comen_US
dc.identifier.citationSelvakumar N, Ravikumar D, Sivagamasundari S, Gopi PG, Narayanan PR. A novel method of staining acid-fast bacilli in sputum containers. Indian Journal of Medical Research. 2006 Jun; 123(6): 776-80en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/22782
dc.language.isoengen_US
dc.source.urihttps://icmr.nic.in/ijmr/ijmr.htmen_US
dc.subject.meshCentrifugationen_US
dc.subject.meshHumansen_US
dc.subject.meshMicroscopyen_US
dc.subject.meshMycobacterium --isolation & purificationen_US
dc.subject.meshRosaniline Dyesen_US
dc.subject.meshSpecimen Handling --methodsen_US
dc.subject.meshSputum --microbiologyen_US
dc.subject.meshStaining and Labeling --methodsen_US
dc.subject.meshTuberculosis, Pulmonary --diagnosisen_US
dc.titleA novel method of staining acid-fast bacilli in sputum containers.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
dc.typeResearch Support, U.S. Gov't, Non-P.H.S.en_US
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