Neuroprotective Effect of Convolvulus pluricaulis Methanol Extract on Hydrogen Peroxide Induced Oxidative Stress in Human IMR32 Neuroblastoma Cell Line.
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Date
2012-10
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Abstract
Aims: The present study aimed to evaluate and ascertain the protective role of
methanolic/ethanolic/water extracts of Convolvulus pluricaulis against H2O2 induced
cytotoxicity in IMR32 Neuroblastoma cell line as model system and identify the factor
responsible for the protective effect.
Study Design: Experimental study.
Place and Duration of Study: Department of Molecular Biology and Biochemistry, Guru
Nanak Dev University, Amritsar & Department of Biotechnology, DAV College, Amritsar,
PuCPab, between August 2010 and March 2012.
Methodology: Firstly, cytotoxic dose of H2O2 and non-toxic dose of methanolic, ethanolic
and water extracts of C. pluricaulis (CP-MEx, CP-EEx and CP-WEx respectively) was
determined by MTT assay. Protective effect of CP-MEx, CP-EEx and CP-WEx was
determined using quercetin as a positive control. The expression of IMR32 cytoskeletal
marker, Neurofilament (NF-200) and stress markers, Heat shock protein (HSP70) and (glucose regulated protein 75, Grp75) Mortalin studied by immunofluorescence and RTPCR
results. The level of antioxidant enzymes catalase, superoxide dismutase,
glutathione peroxidase, direct scavenger of free radicals, Glutathione and lipid
peroxidation were analysed by their standard procedures.
Results: The results showed that quercetin, CP-MEx, CP-EEx and CP-WEx displayed
cytoprotective activity in IMR32 cells. Out of tested extracts CP-MEx significantly
decreased hydrogen peroxide-induced cell death. Significant decrease in NF-200, HSP70
and Mortalin expression was observed in CP-MEx+H2O2 treated cultures as compared to
H2O2 treated. Catalase, superoxide dismutase, glutathione peroxidase, Glutathione levels
significantly increased in Quercetin and CP-MEx treated cultures. Lipid peroxidation was
significantly decreased in both Quercetin and CP-MEx treated cultures.
Conclusions: The present work establishes the protective effect of CP-MEx on IMR 32
Human Neuroblastoma cell line which is as much as by quercetin. The cytoprotective
effect of CP-MEx was due to induction of antioxidant machinery of the cell hence holds
therapeutic value in the treatment and/or prevention of neurodegenerative disorders of
oxidative stress.
Description
Keywords
Convolvulus pluricaulis, IMR32 human neuroblastoma, hydrogen peroxide, antioxidnat enzymes, NF-200, HSP70, mortalin
Citation
Dhuna Kshitija, Dhuna Vikram, Bhatia Gaurav, Singh Jatinder, Kamboj Sukhdev Singh. Neuroprotective Effect of Convolvulus pluricaulis Methanol Extract on Hydrogen Peroxide Induced Oxidative Stress in Human IMR32 Neuroblastoma Cell Line. British Biotechnology Journal. 2012 Oct; 2(4): 192-210.