Role of cyclooxygenase-2 in lipopolysaccharide-induced hyperalgesia in formalin test.

Simple item page
dc.contributor.authorPadi, Satyanarayana S Ven_US
dc.contributor.authorKulkarni, Shrinivas Ken_US
dc.date.accessioned2009-05-28T15:50:09Z
dc.date.available2009-05-28T15:50:09Z
dc.date.issued2005-01-05en_US
dc.description.abstractLipopolysaccharide (LPS)-induced hyperalgesia and the role of cyclooxygenase (COX) isoforms in acute and chronic nociceptive assays have been well established. However, the role of COX isoforms in LPS-induced hyperalgesia in the formalin test is not clear. Thus, the present study was undertaken to characterize the time course of formalin-induced nociceptive response in LPS-pretreated mice and to investigate possible effects of COX inhibitors to address the potential role of COX isoforms in LPS-induced hyperalgesia in the formalin test. All the animals showed typical biphasic response to formalin challenge. At 0 hr (immediately) and 4 hr after LPS pretreatment, animals did not show any alteration in formalin-induced tonic pain. However, 12 and 16 hr after LPS pretreatment, there was a significant increase in the late phase of formalin-induced nocifensive response as compared to control mice. Treatment with intravenously administered ketorolac (a nonselective COX inhibitor) significantly and dose-dependently inhibited the late phase of formalin-induced nociceptive behaviour in saline and LPS-pretreated mice. In contrast, parecoxib (prodrug of valdecoxib, a selective COX-2 inhibitor) or dexamethasone (COX-2 transcription inhibitor), when administered intravenously or intraperitoneally, respectively, did not show antinociceptive effect in the formalin test in saline-pretreated mice. However, both the agents significantly and dose-dependently decreased the late phase nociceptive behaviour of the formalin test in LPS-pretreated mice to the level of the animals that received saline pretreatment. These results suggest that induction of COX-2 by proinflammatory mediators and subsequent release of prostaglandins could be responsible for LPS enhancement of formalin-induced nocifensive behaviour and supports an important role of COX-2 in LPS-induced hyperalgesia in the formalin test.en_US
dc.description.affiliationUniversity Institute of Pharmaceutical Sciences, Panjab University, Chandigarh 160014, India.en_US
dc.identifier.citationPadi SS, Kulkarni SK. Role of cyclooxygenase-2 in lipopolysaccharide-induced hyperalgesia in formalin test. Indian Journal of Experimental Biology. 2005 Jan; 43(1): 53-60en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/62474
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCyclooxygenase 2en_US
dc.subject.meshCyclooxygenase 2 Inhibitorsen_US
dc.subject.meshCyclooxygenase Inhibitors --therapeutic useen_US
dc.subject.meshDexamethasone --therapeutic useen_US
dc.subject.meshDisease Models, Animalen_US
dc.subject.meshFemaleen_US
dc.subject.meshHyperalgesia --chemically induceden_US
dc.subject.meshIsoenzymes --metabolismen_US
dc.subject.meshIsoxazoles --therapeutic useen_US
dc.subject.meshKetorolac --therapeutic useen_US
dc.subject.meshLipopolysaccharides --toxicityen_US
dc.subject.meshMaleen_US
dc.subject.meshMiceen_US
dc.subject.meshPain Measurementen_US
dc.subject.meshProstaglandin-Endoperoxide Synthases --metabolismen_US
dc.subject.meshSalmonella typhimurium --metabolismen_US
dc.titleRole of cyclooxygenase-2 in lipopolysaccharide-induced hyperalgesia in formalin test.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
Files
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.79 KB
Format:
Plain Text
Description:
Download
Collections
Indian Journal of Experimental Biology