Strain improvement of entomopathogenic fungal species beauveria bassiana and metarhizium anisopliae by protoplast fusion.
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Date
2010-11
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Abstract
Protease hyper producing recombinant strains were produced by intergeneric
protoplast fusion of entomopathogenic fungi Beauveria bassiana and
Metarhizium anisopliae. β-glucuronidase and KCl were used as lysing enzyme and
osmotic stabilizer. Along with inter-generic fusion, intra-strain and inter-strain fusions
were also carried out using polyethylene glycol (PEG) as fusogen. When the fused
protoplasts were regenerated on Czapekdox agar medium, they exhibited fast mycelial
growth and abundant sporulation when compared to non-fusants. Pr1 and Pr2 specific
activities were found to be increased by two- fold in recombinant strains than the nonfusants
and the parental strains.
Description
Keywords
Entomopathogenic fungi, Beauveria bassiana, Metarhizium anisopliae, Protoplast isolation, Protoplast fusion
Citation
Sirisha S, Kaur S Gurvinder, Palem P C Padmini. Strain improvement of entomopathogenic fungal species beauveria bassiana and metarhizium anisopliae by protoplast fusion. International Journal of Applied Biology and Pharmaceutical Technology. 2010 Nov-Dec; 1(3): 1135-1143.