Correlation of Helicobacter pylori virulence genotype & severity of mucosal inflammation in gastric biopsies from two geographically diverse regions in India

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dc.contributor.authorSharma, Nen_US
dc.contributor.authorDas, Pen_US
dc.contributor.authorDas, Ren_US
dc.contributor.authorMahant, Sen_US
dc.contributor.authorKalaivani, Men_US
dc.contributor.authorYadav, Ren_US
dc.contributor.authorRajeshwari, Men_US
dc.contributor.authorKedia, Sen_US
dc.contributor.authorMakharia, GKen_US
dc.contributor.authorSaraya, Aen_US
dc.contributor.authorGupta, SDen_US
dc.contributor.authorAhuja, V.en_US
dc.date.accessioned2023-08-10T07:32:55Z
dc.date.available2023-08-10T07:32:55Z
dc.date.issued2022-09
dc.description.abstractBackground: H. pylori-associated gastritis in patients from the high-altitude area of Ladakh showed severe gastritis, mucosal nodularity, atrophy, and cancer in comparison to those from North India. This study served to analyze if differences in the H. pylori virulence genotypes decide the extent of gastric mucosal inflammation. Methods: Fifty gastric biopsies each from patients with H. pylori-associated gastritis from Ladakh and a tertiary care center in North India were included. The presence of H. pylori strain was confirmed with Warthin starry stain and polymerase chain amplification of the H. pylori-specific 16S rRNA. The cagA, vacA s1, s2, and m1, m2 alleles, and dupA virulence genotypes were studied in all archival samples, followed by their histological correlations. Results: cagA (P 0.009) and vacAs1 m1 (P 0.009) genes were distinctly more in H. pylori strains colonizing the biopsies of North Indian patients. In contrast, the cagA -ve vacAs2 m2 strains were significantly more in H. pylori strain colonizing the biopsies from Ladakhi patients. dupA genotype was almost similarly present in strains from both regions. Among these, only cagA and dupA virulence genes were associated with severe mucosal neutrophilic activity and deep infiltration of H. pylori strains in North Indian patients. Conclusions: Differences in virulence genotypes of H. pylori in gastric biopsies from North Indian and Ladakhi patients were found not significant in deciding the severity of H. pylori-associated gastritis.en_US
dc.identifier.affiliationsDepartment of Pathology, All India Institute of Medical Sciences, New Delhi, Indiaen_US
dc.identifier.affiliationsCentre for Medical Biotechnology, Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh, Indiaen_US
dc.identifier.affiliationsDepartment of Biostatistics, All India Institute of Medical Sciences, New Delhi, Indiaen_US
dc.identifier.affiliationsDepartment of Gastroenterology and Human Nutrition, All India Institute of Medical Sciences, New Delhi, Indiaen_US
dc.identifier.citationSharma N, Das P, Das R, Mahant S, Kalaivani M, Yadav R, Rajeshwari M, Kedia S, Makharia GK, Saraya A, Gupta SD, Ahuja V.. Correlation of Helicobacter pylori virulence genotype & severity of mucosal inflammation in gastric biopsies from two geographically diverse regions in India. Indian Journal of Pathology & Microbiology. 2022 Sept; 65(3): 535-544en_US
dc.identifier.issn0377-4929
dc.identifier.issn0974-5130
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/223326
dc.languageenen_US
dc.publisherWolters Kluwer - Medknowen_US
dc.relation.issuenumber3en_US
dc.relation.volume65en_US
dc.source.urihttps://doi.org/10.4103/ijpm.ijpm_565_21en_US
dc.subjectGastric biopsyen_US
dc.subjectgenotypeen_US
dc.subjectHelicobacter pylorien_US
dc.subjecthistologyen_US
dc.subjectLadakhen_US
dc.subjectNorth Indiaen_US
dc.titleCorrelation of Helicobacter pylori virulence genotype & severity of mucosal inflammation in gastric biopsies from two geographically diverse regions in Indiaen_US
dc.typeJournal Articleen_US
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